DDB1- and CUL4-associated factor 11 (DCAF11)
The protein contains 546 amino acids for an estimated molecular weight of 61670 Da.
May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs3825584 |
Biological Process
Post-translational protein modification
Proteasome-mediated ubiquitin-dependent protein catabolic process
Protein ubiquitination
Molecular Function
The reference OMIM entry for this protein is 613317
Ddb1- and cul4-associated factor 11; dcaf11
Wd repeat-containing protein 23; wdr23
CLONING
By searching a database for sequences similar to C. elegans Wdr23, Choe et al. (2009) identified 2 splice variants of human DCAF11, which they called WDR23. The deduced 546- and 520-amino acid WDR23 isoforms differ only in the presence or absence of a 26-amino acid insert near the N terminus. Both isoforms contain 7 WD40 repeats and a conserved DW box motif that overlaps WD40 repeat 5.GENE FUNCTION
The transcription factor Skn1 (POU2F3; 607394) protects C. elegans from stress and promotes longevity. Choe et al. (2009) showed that Wdr23 interacted with Skn1 and Ddb1 (600045) in C. elegans. Loss of Wdr23 function caused accumulation of Skn1 in intestinal nuclei, elevation of Skn1 protein levels, constitutive transcription of detoxification genes, and increased longevity and stress resistance. Wdr23 interacted with the Ddb1-Cul4 (see CUL4A; 603137) complex and presumably targeted Skn1 for proteasomal degradation. Choe et al. (2009) concluded that Wdr23 is a negative regulator of Skn1 in C. elegans.MAPPING
Hartz (2010) mapped the DCAF11 gene to chromosome 14q11.2 based on an alignment of the DCAF11 sequence (GenBank GENBANK AF130070) with the genomic sequence (GRCh37). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 613317 was added.