Signal transducing adapter molecule 1 (STAM)
The protein contains 540 amino acids for an estimated molecular weight of 59180 Da.
Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| a colorectal cancer sample |
Biological Process
Endosomal transport
Epidermal growth factor receptor signaling pathway
Intracellular protein transport
Macroautophagy
Membrane organization
Multivesicular body assembly
Negative regulation of epidermal growth factor receptor signaling pathway
Positive regulation of exosomal secretion
Positive regulation of signal transduction
Protein deubiquitination
Protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
Regulation of extracellular exosome assembly
Signal transduction
Cellular Component
Cytosol
Early endosome membrane
ESCRT-0 complex
Intracellular membrane-bounded organelle
The reference OMIM entry for this protein is 601899
Signal-transducing adaptor molecule 1; stam
Signal-transducing adaptor molecule
Stam1
CLONING
Stimulation of cells with cytokines results in a signal transduction cascade involving cytokine receptors, Janus kinases (JAKs) and signal transducers and activators of transcription (STATs). In order to investigate signal transduction downstream of JAK3 (600173), Takeshita et al. (1996) screened for molecules induced after stimulation of cells with the cytokine IL2 (147680). Their screen identified a novel molecule, which they named STAM for 'signal-transducing adaptor molecule.' They cloned the human STAM cDNA from a T-cell cDNA library and found that it encodes a 540-amino acid polypeptide. The approximately 70-kD protein product was precipitated by anti-phosphotyrosine. Northern blot analysis indicated that STAM was expressed as a 2.9-kb message in all tissue and cell types examined. The STAM sequence contains a Src-homology 3 (SH3) domain and an immunoreceptor tyrosine-based activation motif (ITAM). Takeshita et al. (1996) suggested that STAM acts as an adaptor molecule in signal transduction pathways from cytokine receptors.GENE FUNCTION
Asao et al. (1997) showed that HGS (604375) binds to STAM via coiled-coil sequences and appears to regulate proliferation in response to cytokines.MAPPING
Takeshita et al. (1996) used fluorescence in situ hybridization to map the STAM gene to human chromosome 10p14-p13. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 601899 was added.
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed