Perilipin-3 (PLIN3)
The protein contains 434 amino acids for an estimated molecular weight of 47075 Da.
Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs8289 | |
| dbSNP:rs9973235 |
Biological Process
Lipid storage
Positive regulation of sequestering of triglyceride
Vesicle-mediated transport
The reference OMIM entry for this protein is 602702
Perilipin 3; plin3
Mannose 6-phosphate receptor-binding protein 1; m6prbp1
Mpr-binding protein, 47-kd
Mpr tail-interacting protein, 47-kd; tip47
CLONING
Mannose 6-phosphate receptors (MPRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. Using yeast 2-hybrid analysis to identify proteins that interact with the cytoplasmic domains of cation-independent MPR (IGF2R; 147280) and cation-dependent MPR (M6PR; 154540), Diaz and Pfeffer (1998) cloned TIP47. The deduced protein contains 434 amino acids. Immunoblot analysis showed that it had an apparent molecular mass of 47 kD.GENE FUNCTION
Diaz and Pfeffer (1998) showed that TIP47 bound selectively to the cytoplasmic domains of both cation-independent and cation-dependent MPRs. TIP47 was present in cytosol and on endosomes and was required for MPR transport from endosomes to the trans-Golgi network in vitro and in vivo. TIP47 recognized a phenylalanine/tryptophan signal in the tail of cation-dependent MPR essential for its proper sorting within the endosomal pathway. These data suggested that TIP47 binds MPR cytoplasmic domains and facilitates their collection into transport vesicles destined for the Golgi. TIP47 recognizes the cytoplasmic domains of MPRs and is required for endosome-to-Golgi transport. Carroll et al. (2001) demonstrated that TIP47 binds directly to the RAB9 GTPase (300284) in its active, GTP-bound conformation. Moreover, RAB9 increases the affinity of TIP47 for its cargo. A functional RAB9 binding site was required for TIP47 stimulation of MPR transport in vivo. Thus, Carroll et al. (2001) concluded that a cytosolic cargo selection device may be selectively recruited onto a specific organelle, and vesicle budding might be coupled to the presence of an active RAB GTPase. Aivazian et al. (2006) generated a RAB5 (179512)/RAB9 chimera, which could bind both RAB5 and RAB9 effectors, and a RAB1 (179508)/RAB9 chimera, which could bind both RAB1 and RAB9 effectors, and examined the role of effector binding on RAB localization. In both cases, changing the cellular concentration of the RAB9 effector TIP47 moved a fraction of the proteins from their parental RAB localization to that of RAB9. They concluded that effector proteins and RABs rely on one another to achieve correct steady state localizations. By mutation and immunoblot analysis, Lopez-Verges et al. (2006) found that TIP47 acted as a link between the human immunodeficiency virus (HIV)-1 Gag and Env proteins by binding to the matrix domain of Gag and to the cytoplasmic tail of the transmembrane gp41 subunit of Env. Silencing of TIP47 impaired incorporation of Env into Gag particles as well as HIV-1 infectivity, and it abolished coimmunoprecipitation of Gag and Env. In contrast, overexpression of TIP47 enhanced incorporation of Env into virions. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 602702 was added.