Serine/threonine-protein kinase 38-like (STK38L)
The protein contains 464 amino acids for an estimated molecular weight of 54003 Da.
Involved in the regulation of structural processes in differentiating and mature neuronal cells. (updated: Jan. 7, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| a aLL TEL/AML1+ sample; somatic mutation |
The reference OMIM entry for this protein is 615836
Serine/threonine protein kinase 38-like protein; stk38l
Stk38-like protein
Nuclear dbf2-related protein 2; ndr2
Kiaa0965
CLONING
By sequencing clones obtained from a size-fractionated human brain cDNA library, Nagase et al. (1999) cloned STK38L, which they designated KIAA0965. The deduced 463-amino acid protein shares significant similarity with human NDR protein kinase (STK38; 606964). RT-PCR ELISA detected low to moderate expression in all human and fetal tissues and specific adult bran regions examined except liver and spleen, which showed little to no expression. By RT-PCR of total HeLa cell RNA, Devroe et al. (2004) cloned STK38L, which they called NDR2. The deduced 461-amino acid protein shares approximately 87% identity with NDR1 (STK38). Both proteins have an N-terminal S100B (176990)-binding domain and 3 putative regulatory phosphorylation sites. Northern blot analysis detected NDR2 expression in all 10 human tissues examined, with highest expression in thymus. Epitope- and fluorescence-tagged NDR2 was expressed in cytoplasm in a punctate distribution. Stegert et al. (2004) cloned mouse Ndr2. Both human and mouse NDR2 contain 464 amino acids and have calculated molecular masses of 54 kD. They share 97% amino acid identity. Real-time PCR of 14 mouse tissues detected high Ndr2 expression in large intestine, small intestine, stomach, and testis, with little to no expression in other tissues.GENE FUNCTION
By coimmunoprecipitation and mass spectrometric analysis, Devroe et al. (2004) found that both epitope-tagged NDR1 and NDR2 interacted with MOB2 (HCCA2; 611969) and that epitope-tagged NDR2 interacted with MOB1B (MOBKL1A; 609282) in Jurkat human T-cell lysates. MOB2 stimulated autophosphorylation of NDR2 and, to a lesser extent, NDR1 in in vitro kinase reactions. NDR2 phosphorylated MBP (159430) and 3 contaminants in the MBP preparation. Stegert et al. (2004) found that treatment of transfected COS-1 cells with okadaic acid resulted in phosphorylation of mouse Ndr2 on thr75, ser282, and thr444. Ser282 was the site of autophosphorylation. Stimulation by calcium/S100B induced Ndr2 phosphorylation and activity in a concentration-dependent manner.GENE STRUCTURE
Stegert et al. (2004) determined that the human and mouse STK38L genes contain 14 exons. Exon 1 is noncoding.MAPPING
By radiation hybrid analysis, Nagase et al. (1999) mapped the STK38L gene to chromosome 12. By genomic sequence analysis, Stegert et al. (2004) mapped the STK38L gene to chromosome 12p12.3. They mapped the mouse Stk38L gene to a region of chromosome 6G2-G3 that shares homology of synteny with human chromosome 12p12.3. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 615836 was added.