Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-7 (GNG7)
The protein contains 68 amino acids for an estimated molecular weight of 7522 Da.
Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction. Plays a role in the regulation of adenylyl cyclase signaling in certain regions of the brain. Plays a role in the formation or stabilzation of a G protein heterotrimer (G(olf) subunit alpha-beta-gamma-7) that is required for adenylyl cyclase activity in the striatum (By similarity). (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt.
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Biological Process
Behavioral fear response
G protein-coupled receptor signaling pathway
Locomotory behavior
Receptor guanylyl cyclase signaling pathway
Regulation of adenylate cyclase activity
Regulation of G protein-coupled receptor signaling pathway
The reference OMIM entry for this protein is 604430
Guanine nucleotide-binding protein, gamma-7; gng7
See 600874 for background. Using the differential display technique, Shibata et al. (1998) investigated genes differentially expressed in normal, as compared to cancerous, tissues of the pancreas. They identified a cDNA fragment that was expressed in normal tissue but was rarely expressed in cancer tissue. They isolated a cDNA encoding a 68-amino acid protein from a normal pancreatic tissue cDNA library using 5-prime RACE. The inferred amino acid sequence encoded a G protein gamma subunit with 98% homology to cow G protein gamma-7 and complete homology to human G protein gamma-7, identified by Ray et al. (1995). Decreased expression of G protein gamma-7 in pancreatic cancer was confirmed by Northern blot assay in 12 pancreatic malignancies, including 9 duct cell carcinomas, 2 cystoadenocarcinomas, and 1 blastoma. RT-PCR assay showed no expression of G protein gamma-7 in 5 of 6 pancreatic carcinoma cell lines and 2 pancreatic cancer tissues. Immunohistochemical analysis displayed positive staining in normal tissue but no staining in cancer tissue. Shibata et al. (1998) suggested that reduced or suppressed expression of G protein gamma-7 may play an important role in pancreatic carcinogenesis. ... More on the omim web site
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 604430 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).