Carbonyl reductase [NADPH] 3 (CBR3)
The protein contains 277 amino acids for an estimated molecular weight of 30850 Da.
Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro). (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Molecular Function
3-keto sterol reductase activity
Carbonyl reductase (NADPH) activity
NADPH binding
The reference OMIM entry for this protein is 603608
Carbonyl reductase 3; cbr3
DESCRIPTION
Carbonyl reductase (CBR; EC 1.1.1.184) catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols.CLONING
By analyzing sequences from the 21q22.2 chromosomal region, Watanabe et al. (1998) identified genomic clones corresponding to CBR1 (114830) and to CBR3, a novel carbonyl reductase gene. The deduced 277-amino acid CBR3 protein is 84% similar to the CBR1 protein.GENE STRUCTURE
The CBR3 gene contains 3 exons spanning 11.2 kb (Watanabe et al., 1998).MAPPING
Watanabe et al. (1998) found that the CBR3 gene is located 62 kb telomeric of CBR1 on chromosome 21q22.2. ... More on the omim web site
Oct. 19, 2018: Protein entry updated
Automatic update: OMIM entry 603608 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).