The reference OMIM entry for this protein is 164880

V-yes-1 yamaguchi sarcoma viral oncogene; yes1
Oncogene yes1
Yamaguchi sarcoma oncogene

DESCRIPTION

The YES1 oncogene is homologous to the v-yes gene of the Yamaguchi sarcoma virus. The v-yes gene product is associated with tyrosine-specific protein kinase activity, and its amino acid sequence shows a high degree of homology with that of the v-src gene product of Rous sarcoma virus (see SRC; 190090) (summary by Semba et al., 1985).

CLONING

Using Northern blot analysis, Semba et al. (1985) found variable expression of a 4.8-kb RNA related to v-yes in human embryonic fibroblasts, 3 human cell lines, placenta, and fetal lung, liver, and kidney. Using a probe prepared from human YES2, a processed pseudogene, Sukegawa et al. (1987) cloned human YES1 from an embryonic fibroblast cDNA library. The deduced 543-amino acid protein has a calculated molecular mass of 60.8 kD. It has a putative kinase domain, conserved ATP-binding residues (lys305 and a GxGxxG motif at residue 284), a putative autophosphorylation site (tyr416), and a putative myristoylation site (gly2). Amino acids 91 through 543 of human YES1, including the kinase domain, share 96% identity with the corresponding region of v-yes. Oh et al. (2002) reported that the deduced 541-amino acid YES1 protein contains an SRC homology-3 (SH3) domain, followed by an SH2 domain and a C-terminal protein tyrosine kinase domain.

GENE FUNCTION

Using a yeast 2-hybrid assay of a HeLa cell library, Oh et al. (2002) showed that YES1 interacted with QM (RPL10; 312173). Immunoprecipitation analysis and protein pull-down assays showed that QM interacted with the SH3 domain of YES1. Full-length QM suppressed the kinase activity of YES1 by suppressing its autophosphorylation activity. Overexpression of QM resulted in increased YES1 mRNA and protein expression. Taniguchi et al. (2015) showed in mice and human cells that GP130 (600694), a coreceptor for IL6 (147620) cytokines, triggers activation of YAP (606608) and Notch (190198), transcriptional regulators that control tissue growth and regeneration, independently of the GP130 effector STAT3 (102582). Through YAP and Notch, intestinal GP130 signaling stimulates epithelial cell proliferation, causes aberrant differentiation, and confers resistance to mucosal erosion. GP130 associates with the related tyrosine kinases SRC (190090) and YES, which are activated on receptor engagement to phosphorylate YAP and induce its stabilization and nuclear translocation. This signaling module is strongly activated upon mucosal injury to promote healing and maintain barrier function.

GENE STRUCTURE

Sukegawa et al. (1987) found that the upstream region of the YES1 gene is GC rich.

MAPPING

Semba et al. (1985) found in DNA from human embryo fibroblasts 10 EcoRI fragments that hybridized with the Yamaguchi sarcoma virus oncogene. By a study of human-mouse cell hybrids, 4 of these fragments, designated YES1, were assigned to chromosome 18, and 1 fragment, designated YES2, was assigned to chromosome 6. (YES2 was later found by Semba et al. (1988) to be a pseudogene of YES1 and to be located at chromosome 22q11.2. Semba et al. (1988) stated: 'The failure of proper mapping in our earlier experiment might have been caused by instability of hybrid cell clones.') The other 5 fragments could not be mapped either because hybridization signals were too weak or differentiation from mouse Yes fragments was impossible. There was evidence for multiple copies of YES-related genes in the human genome. At least 3 of the human ... More on the omim web site

Subscribe to this protein entry history

Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 164880 was added.

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).