Protein transport protein Sec31A (SEC31A)

The protein contains 1220 amino acids for an estimated molecular weight of 133015 Da.

 

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER) (PubMed:10788476). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules (By similarity). (updated: Jan. 31, 2018)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

Interpro domains
Total structural coverage: 0%
Model score: 0
No model available.

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VariantDescription
dbSNP:rs34554214
dbSNP:rs3797036
dbSNP:rs35579207
dbSNP:rs35739017

The reference OMIM entry for this protein is 610257

Sec31, yeast, homolog of, a; sec31a
Sec31-like 1; sec31l1
Kiaa0905

DESCRIPTION

SEC31A is a subunit of coat protein complex II (COPII)-coated vesicles, which are essential for secretion (Jin et al., 2012).

CLONING

By sequencing clones obtained from a size-fractionated adult brain cDNA library, Nagase et al. (1998) cloned SEC31L1, which they designated KIAA0905. The deduced protein contains 1,220 amino acids. RT-PCR detected moderate expression in all adult and fetal tissues and specific brain regions examined. By searching EST databases for sequences similar to yeast Sec31, followed by screening a pancreas cDNA library, Tang et al. (2000) cloned SEC31L1, which they called SEC31A. The deduced 1,218-amino acid protein shares 25.8% identity with yeast Sec31. It contains 5 WD40 or WD40-like repeats at its N terminus and a proline-rich region in its C-terminal half. Northern blot analysis detected a 4-kb transcript that was abundantly and ubiquitously expressed. In rat kidney cells, Sec31a colocalized with Sec13 (SEC13L1; 600152) in vesicular-tubular structures characteristic of endoplasmic reticulum (ER) exit sites.

GENE FUNCTION

By immunostaining for Sec31a in intact and permeabilized rat kidney cells, Tang et al. (2000) found that Sec31a was not tightly associated with the membrane. Binding of Sec31a to specific membrane structures was restored by incubating washed cells with cytosol, indicating that Sec31a was recruited to membranes. The membrane association of Sec31a was greatly enhanced in the presence of a nonhydrolyzable GTP analog. Tang et al. (2000) demonstrated that Sec31A and Sec13 coimmunoprecipitated and that the proteins existed in a 600- to 700-kD complex. Immunodepletion studies showed that rat Sec31a was required for ER-to-Golgi vesicular transport. Jin et al. (2012) found that monoubiquitination of Sec31 in mouse embryonic stem cells by Klhl12 (614522) and the Cul3 (603136) E3 ubiquitin ligase complex was required for COPII vesicle expansion to accommodate large cargo proteins, such as procollagens (see 120150). A Sec31-binding mutant of Klhl12 neither colocalized with Sec31 at intracellular vesicles nor induced formation of large vesicles. Disruption of KLHL12-CUL3 function in human HT1080 fibrosarcoma cells impaired COPII vesicle expansion and collagen export, but it had no effect on export of smaller cargo by small COPII vesicles. Jin et al. (2012) concluded that KLHL12-CUL3 monoubiquitination of SEC31 is required for COPII vesicle expansion to accommodate large or bulky cargo molecules.

MAPPING

By radiation hybrid analysis, Nagase et al. (1998) mapped the SEC31L1 gene to chromosome 4. ... More on the omim web site

Subscribe to this protein entry history

Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 16, 2016: Protein entry updated
Automatic update: OMIM entry 610257 was added.