Intercellular adhesion molecule 3 (ICAM3)
The protein contains 547 amino acids for an estimated molecular weight of 59541 Da.
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2) (PubMed:1448173). ICAM3 is also a ligand for integrin alpha-D/beta-2. In association with integrin alpha-L/beta-2, contributes to apoptotic neutrophil phagocytosis by macrophages (PubMed:23775590). (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is predicted to be membranous by TOPCONS.
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| Variant | Description |
|---|---|
| dbSNP:rs17697947 | |
| dbSNP:rs7258015 | |
| dbSNP:rs2304237 | |
| dbSNP:rs2230399 |
The reference OMIM entry for this protein is 146631
Intercellular adhesion molecule 3; icam3
CLONING
Fawcett et al. (1992) cloned a third ligand for LFA1 (153370; 600065); also see intercellular adhesion molecules 1 and 2 (ICAM1, 147840; ICAM2, 146630). ICAM1 and ICAM2 are members of the immunoglobulin superfamily. The interaction of LFA1 with ICAM provides essential accessory adhesion signals in many immune interactions, including those between T and B lymphocytes and cytotoxic T cells and their targets. Both ICAMs are expressed at low levels on resting vascular endothelium; ICAM1 is strongly upregulated by cytokine stimulation and plays a key role in the arrest of leukocytes in blood vessels at sites of inflammation and injury. Resting leukocytes were shown to express a third ligand, ICAM3, for LFA1. ICAM3 is potentially the most important ligand for LFA1 in the initiation of the immune response because the expression of ICAM1 on resting leukocytes is low. Fawcett et al. (1992) used expression cloning to isolate a cDNA encoding a protein which is constitutively expressed on all leukocytes and which binds LFA1. ICAM3 is closely related to ICAM1, consists of 5 immunoglobulin domains, and binds LFA1 through its 2 N-terminal domains. Vazeux et al. (1992) cloned the same intercellular adhesion molecule, which they referred to as ICAM-R. The cDNA was 1,781 bp long, with a 1,640-bp open reading frame starting with an ATG at position 16, followed by a second ATG at position 24. Overall identities in protein structure with ICAM1 and ICAM2 were 48% and 31%, respectively, with the degree of similarity varying between individual domains.MAPPING
Using the cDNA as a probe, Bossy et al. (1994) mapped the ICAM3 gene to 19p13.3-p13.2 by isotopic in situ hybridization. They also mapped the gene to chromosome 19 by PCR amplification of DNAs from somatic cell hybrids. ICAM1 maps to the same region of chromosome 19. ... More on the omim web site
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 146631 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).