Sodium/potassium-transporting ATPase subunit beta-3 (ATP1B3)
The protein contains 279 amino acids for an estimated molecular weight of 31513 Da.
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The exact function of the beta-3 subunit is not known. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt, is predicted to be membranous by TOPCONS.
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No binding partner found
Biological Process
Cellular potassium ion homeostasis
Cellular sodium ion homeostasis
Ion transmembrane transport
Leukocyte migration
Membrane repolarization
Positive regulation of ATPase activity
Positive regulation of potassium ion import across plasma membrane
Positive regulation of potassium ion transmembrane transporter activity
Positive regulation of sodium ion export across plasma membrane
Potassium ion import across plasma membrane
Potassium ion transmembrane transport
Protein localization to plasma membrane
Protein stabilization
Regulation of cardiac conduction
Sodium ion export across plasma membrane
Sodium ion transmembrane transport
The reference OMIM entry for this protein is 601867
Atpase, na+/k+ transporting, beta-3 polypeptide; atp1b3
Na,k-atpase beta-3 polypeptide
DESCRIPTION
The Na+/K+ ATPase is a plasma membrane pump with numerous physiologic functions. It maintains ionic homeostasis that is critical for cell survival, differentiation, and apoptosis. The Na+/K+ ATPase holoenzyme consists of a catalytic alpha subunit (see 182310), a beta subunit, and a modulatory gamma subunit (FXYD2; 601814). Beta subunits, such as ATP1B3, are responsible for formation and structural integrity of the Na+/K+ ATPase holoenzyme (summary by Li et al., 2011).CLONING
Malik et al. (1996) found the human homolog of the Xenopus Na,K-ATPase beta-3 gene in the EST database. They sequenced this human cDNA and its rat ortholog. The human gene, designated ATP1B3, encodes a 279-amino acid polypeptide with 1 putative transmembrane domain. Northern blot analysis of rat tissues revealed that the ATP1B3 gene encodes 2 transcripts of 1.6 and 1.8 kb that are expressed in a variety of tissues, with highest expression in testis.GENE STRUCTURE
Malik et al. (1998) reported that the ATP1B3 gene is more than 50 kb long and contains 7 exons. Identical exon/intron organization of the ATP1B3 gene and the ATP1B2 gene (182331) suggested that the genes evolved from a common evolutionary ancestor.MAPPING
By fluorescence in situ hybridization with PAC DNA clones, Malik et al. (1998) localized the ATP1B3 gene to chromosome 3q22-q23 and a pseudogene to 2p15-p13. Gross (2014) mapped the ATP1B3 gene to chromosome 3q23 based on an alignment of the ATP1B3 sequence (GenBank GENBANK BC011835) with the genomic sequence (GRCh37). Besirli et al. (1998) mapped the Atp1b3 gene to mouse chromosome 9 and a related gene to mouse chromosome 3. The mapping was accomplished by haplotype analysis of 94 progeny from an interspecific backcross.HISTORY
Malik et al. (1996) used RFLP mapping to localize the Atp1b3 gene to mouse chromosome 7, with the highest probability of its location being 6.2 cM distal to Gpi1 and 12 cM proximal to Tam1. Malik et al. (1998) suggested that the assignment of the Atp1b3 gene to mouse chromosome 7 by Malik et al. (1996) may have been in error or may have represented the mapping of the mouse pseudogene. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 601867 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).