Epithelial membrane protein 3 (EMP3)
The protein contains 163 amino acids for an estimated molecular weight of 18429 Da.
Probably involved in cell proliferation and cell-cell interactions. (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is predicted to be membranous by TOPCONS.
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| Variant | Description |
|---|---|
| dbSNP:rs4893 |
No binding partner found
Molecular Function
The reference OMIM entry for this protein is 602335
Epithelial membrane protein 3; emp3
CLONING
By homology screening of databases, Ben-Porath and Benvenisty (1996) and Taylor and Suter (1996) independently identified a cDNA encoding epithelial membrane protein-3 (EMP3). The open reading frame predicts a 163-amino acid protein containing 4 transmembrane domains and 2 N-linked glycosylation sites in the first extracellular loop. Taylor and Suter (1996) showed that the EMP3 cDNA generates an 18-kD protein in vitro. The EMP3 amino acid sequence is 41% and 38% identical to those of peripheral myelin protein-22 (PMP22; 601097) and EMP2 (602334), respectively, with the highest homology found in the transmembrane domains. Due to the high amino acid sequence homology among PMP22, EMP1 (602333), EMP2, and EMP3, Ben-Porath and Benvenisty (1996) stated that these proteins comprise a novel family. Based on the suggested functions of PMP22, Ben-Porath and Benvenisty (1996) and Taylor and Suter (1996) proposed that EMP3 is involved in cell proliferation and cell-cell interactions. Using Northern blot analysis, Taylor and Suter (1996) detected EMP3 expression in all adult tissues examined, with the highest expression in peripheral blood leukocytes and low expression in the brain. In the fetus, they found prominent expression in the lung, liver, and kidney and weak expression in the brain.MAPPING
By FISH, somatic cell hybridization, and radiation hybrid analysis, Liehr et al. (1999) mapped the EMP3 gene to chromosome 19q13.3. Ben Porath et al. (1998) mapped the homologous gene in the mouse to chromosome 7. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 602335 was added.
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).