Cyclin-D1-binding protein 1 (CCNDBP1)
The protein contains 360 amino acids for an estimated molecular weight of 40262 Da.
May negatively regulate cell cycle progression. May act at least in part via inhibition of the cyclin-D1/CDK4 complex, thereby preventing phosphorylation of RB1 and blocking E2F-dependent transcription. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Molecular Function
The reference OMIM entry for this protein is 607089
Cyclin d-type-binding protein 1; ccndbp1
Grap2 cyclin-d-interacting protein; gcip
CLONING
In a yeast 2-hybrid screen with the C-terminal glu/pro-rich domain and the SH3 domain (QC domain) of GRAP2 (604518) as bait, Xia et al. (2000) cloned CCNDBP1, which they called GCIP, from a pretransformed human bone marrow cDNA library. CCNDBP1 encodes a deduced 360-amino acid protein with a calculated molecular mass of 40 kD. It is predicted to contain a helix-loop-helix region without the basic DNA-binding domain, a central acidic domain, and a leucine zipper motif. Northern blot analysis detected a 1.3-kb transcript in all tissues examined, with highest expression in heart, muscle, peripheral leukocytes, kidney, and brain. RT-PCR revealed expression in all immune tissues, with relatively low expression in bone marrow.GENE FUNCTION
Xia et al. (2000) verified interaction between GCIP and the QC domain of GRAP2 in yeast 2 hybrid assays, in in vitro binding assays, and by Western blot analysis of immunoprecipitates of cotransfected COS-7 cells. GST pull-down assays of cell lysates from several cell lines and coimmunoprecipitation of cyclin D with GCIP from GCIP-transfected cells showed that GCIP also associates with cyclin D (CCND1; 168461). Overexpression of GCIP in COS-7 cells reduced the phosphorylation of the retinoblastoma protein (RB1; 614041) by immunopurified cyclin D/CDK4 (123829) complexes. GCIP expression also reduced the transcriptional activity of a cotransfected E2F1 (189971) reporter plasmid.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the CCNDBP1 gene to chromosome 15 (TMAP WI16359). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 607089 was added.
Jan. 28, 2016: Protein entry updated
Automatic update: model status changed
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed