SH3 domain-containing protein 19 (SH3D19)
The protein contains 790 amino acids for an estimated molecular weight of 86525 Da.
May play a role in regulating A disintegrin and metalloproteases (ADAMs) in the signaling of EGFR-ligand shedding. May be involved in suppression of Ras-induced cellular transformation and Ras-mediated activation of ELK1. Plays a role in the regulation of cell morphology and cytoskeletal organization. (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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No binding partner found
The reference OMIM entry for this protein is 608674
Sh3 domain protein 19
Sh3d19
Een-binding protein; ebp sh3d19/runx1 fusion gene, included
CLONING
Using EEN (601768) as bait in a yeast 2-hybrid screen of a HeLa cell cDNA library, Yam et al. (2004) cloned EBP. The deduced 767-amino acid protein has a central proline-rich region and 4 C-terminal SH3 domains. PCR analysis detected EBP expression in all adult and fetal tissues examined. Epitope-tagged EBP was evenly distributed in the cytoplasm of transfected HeLa cells.GENE FUNCTION
Using an in vitro binding assay with several deletion mutants, Yam et al. (2004) showed that the SH3 domain of EEN interacted with the proline-rich motif (PPERP) of EBP. They confirmed the interaction between these proteins by in vitro pull-down assays and coimmunoprecipitation from transfected HeLa cells. Yam et al. (2004) also found that EBP was recruited to the nucleus by the chimeric MLL (159555)-EEN fusion protein. Using yeast 2-hybrid analysis, pull-down assays, and coimmunoprecipitation of transfected HeLa cells, Yam et al. (2004) demonstrated that the SH3 domains of EBP interacted with SOS2 (601247), but not SOS1 (182530), and that the EBP-EEN and EBP-SOS2 interactions occurred simultaneously. Coexpression of EBP with EEN in rat embryonic fibroblasts led to suppression of RAS (190020)-induced cellular transformation and RAS-mediated activation of ELK1 (311040).MAPPING
The International Radiation Hybrid Mapping Consortium mapped the EBP gene to chromosome 4 (TMAP SHGC-31182). Nguyen et al. (2006) identified SH3D19 as a novel translocation partner gene of RUNX1 (151385) in a case of acute myeloid leukemia. The break in chromosome 4 was located at 4q31.3. ... More on the omim web site
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 608674 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).