Inhibitor of nuclear factor kappa-B kinase-interacting protein (IKBIP)
The protein contains 350 amino acids for an estimated molecular weight of 39309 Da.
Target of p53/TP53 with pro-apoptotic function. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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| Variant | Description |
|---|---|
| dbSNP:rs1048906 | |
| dbSNP:rs12322444 |
Cellular Component
Endoplasmic reticulum
Endoplasmic reticulum membrane
Integral component of membrane
Membrane
Molecular Function
The reference OMIM entry for this protein is 609861
I-kappa-b kinase-interacting protein; ikbip
Ikip
Ikk-interacting protein
CLONING
Using yeast 2-hybrid analysis with the NF-kappa-B pathway gene IKK2 (IKBKP: 603258) as bait, Hofer-Warbinek et al. (2004) isolated a novel gene, which they named IKK-interacting protein (IKIP). EST analysis and RT-PCR demonstrated that 3 IKIP transcripts are generated; IKIP1 encodes a 377-amino acid protein, IKIP2 a 350-amino acid protein, and IKIP3 2 potential ORFs encoding 70- and 245-amino acid proteins. IKIP1 harbors a hydrophobic region containing a potential signal anchor sequence and 2 coiled-coil domains; IKIP2 contains a leucine zipper domain, and both IKIP1 and IKIP2 contain a Smc domain. IKIP orthologs are found in other species, including mouse, rat, pig, cow, and Xenopus laevis. Mouse and rat Ikip1 and Ikip2 encode 373- and 345-amino acid proteins, respectively, and the human IKIP1 protein shows 77% and 79% identity with mouse and rat Ikip1, respectively. Northern blot analysis detected expression of 3 IKIP1 transcripts of 1.3, 1.6 and 2.9 kb in lung, kidney, spleen, thymus, and skeletal muscle. Immunofluorescence analysis showed that both transfected and endogenous IKIP1 localizes to the endoplasmic reticulum, and deletion of the hydrophobic region results in uniform distribution of IKIP1 throughout the cell. Tunicamycin and endoglycosidase H treatment demonstrated that IKIP1 and IKIP2 are glycosylated, and targeted mutation of IKIP1 showed that glycosylation occurs at asparagine-154.GENE FUNCTION
Because the transcription start site of IKIP is located 488 bp upstream from the start site of apoptotic protease-activating factor (APAF1; 602233), Hofer-Warbinek et al. (2004) hypothesized that common regulatory elements may exist between these 2 genes. Transfection experiments using p53 (191170) and IKIP1 constructs demonstrated that p53 increases IKIP1 protein levels in a dose-dependent manner. In addition, a variety of experiments looking at endogenous IKIP1 levels showed that IKIP1 expression is dependent on p53. Transfection of IKIP into endothelial cells promotes cell death, as measured by TUNEL staining.GENE STRUCTURE
Hofer-Warbinek et al. (2004) reported that the IKIP gene contains 4 exons spanning 31 kb.MAPPING
Using database analysis, Hofer-Warbinek et al. (2004) found that the IKBIP gene maps to chromosome 12, 488 bp upstream of the transcriptional start site of APAF1. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 609861 was added.
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).