Protein MCM10 homolog (MCM10)
The protein contains 875 amino acids for an estimated molecular weight of 98183 Da.
Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability; acts as a direct target of transcriptional repression by ZBTB38 (PubMed:24726359). (updated: Feb. 20, 2007)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs17152897 | |
| dbSNP:rs34630110 | |
| dbSNP:rs35114749 | |
| dbSNP:rs7905784 | |
| dbSNP:rs2274110 |
The reference OMIM entry for this protein is 609357
Minichromosome maintenance complex component 10; mcm10
Minichromosome maintenance 10, s. cerevisiae, homolog of
Dna43
CLONING
By searching for sequences similar to S. cerevisiae Mcm10, followed by RT-PCR and screening a HeLa cell cDNA library, Izumi et al. (2000) cloned human MCM10. The 3-prime UTR of the transcript contains an Alu repetitive sequence and a poly(A) signal. MCM10 encodes a deduced 874-amino acid protein with a calculated molecular mass of 98 kD. The central region of MCM10, which contains a zinc finger motif, shares significant similarity with the fly, nematode, and yeast proteins. Northern blot analysis detected 5.0- and 3.7-kb transcripts expressed in a 2:1 ratio in both HeLa cells and normal human fibroblasts. Immunolocalization of COS-1 cells transiently expressing MCM10 detected the protein in nuclei in a fine array of foci that were excluded from nucleoli. Western blot analysis detected an endogenous protein of 105 kD in HeLa cells.GENE FUNCTION
Izumi et al. (2000) found that the MCM10 mRNA level increased at the G1/S boundary when quiescent normal human fibroblasts were induced to proliferate with serum. MCM10 associated with nuclease-resistant nuclear structures throughout S phase and dissociated from them in G2 phase. MCM10 associated with ORC2 (ORC2L; 601182) when overexpressed in COS-1 cells, and it interacted with ORC2, MCM2 (116945), and MCM6 (601806) in a yeast 2-hybrid system. Izumi et al. (2004) established HeLa cell lines expressing fluorescence-tagged MCM10. From early to mid-S phase, MCM10 appeared in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus, and in mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In late S and G2 phases, MCM10 localized to nucleoli. Although the distributions of MCM10 during S phase resembled those of replication foci, MCM10 did not colocalize with sites of DNA synthesis in most cases. Furthermore, the transition of MCM10 distribution patterns preceded changes in replication foci patterns or proliferating cell nuclear antigen foci patterns by 30 to 60 minutes. Izumi et al. (2004) concluded that MCM10 is temporarily recruited to the replication sites prior to replication and dissociates from chromatin after activation of the prereplication complex. Ricke and Bielinsky (2004) found that yeast Mcm10 was an essential component of the replication fork and was required to maintain DNA polymerase-alpha (POLA; 180660) on chromatin independently of Cdc45 (603465).MAPPING
By genomic sequence analysis, Izumi et al. (2000) mapped the single-copy MCM10 gene to chromosome 10. ... More on the omim web site
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 609357 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).