Keratin, type II cytoskeletal 73 (KRT73)
The protein contains 540 amino acids for an estimated molecular weight of 58923 Da.
Has a role in hair formation. Specific component of keratin intermediate filaments in the inner root sheath (IRS) of the hair follicle (Probable). (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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No binding partner found
The reference OMIM entry for this protein is 608247
Keratin 73, type ii; krt73
K73
Kb36
Keratin 6, inner root sheath, 3; krt6irs3
K6irs3
DESCRIPTION
K6IRS3 belongs to a family of type II keratins that are specifically expressed in the inner root sheath of hair follicles.CLONING
By searching a sequence database using K6IRS1 (608245) as probe, followed by PCR of a scalp cDNA library, Langbein et al. (2003) cloned full-length K6IRS3. The deduced 540-amino acid protein has a calculated molecular mass of 59 kD. K6IRS3 shares a highly conserved rod domain and flanking non-alpha-helical H1 and H2 subdomains with K6IRS1, K6IRS2 (608246), and K6IRS4 (608248). The head and tail domains are less related. Western blot analysis detected a single K6IRS3 band in root sheath extracts, but not in foot sole extracts. In situ hybridization of plucked beard hair follicles and human scalp showed that K6IRS2 and K6IRS3 were exclusively expressed in the cuticle of the inner root sheath, but they showed different onsets of expression. K6IRS3 was expressed between the lowermost bulb region of the cuticle and the region where Henle cells undergo abrupt terminal differentiation. K6IRS3 protein was detected up to the uppermost cortex region where cuticle cells terminally differentiate. In contrast, K6IRS2 expression was delayed up to the height of the apex of the dermal papilla.MAPPING
By genomic sequence analysis, Langbein et al. (2003) mapped the K6IRS3 gene to chromosome 12q13, within a type II keratin gene cluster. ... More on the omim web site
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 608247 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).