Spermatogenesis-associated protein 33 (SPATA33)
The protein contains 139 amino acids for an estimated molecular weight of 15461 Da.
No function (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs13329897 |
No binding partner found
The reference OMIM entry for this protein is 615409
Spermatogenesis-associated protein 33; spata33
Chromosome 16 open reading frame 55; c16orf55
DESCRIPTION
In mouse, Spata33 is predominantly expressed in juvenile and adult testicular germ cells and is predicted to be involved in spermatogenesis (Chen et al., 2013).CLONING
Chen et al. (2013) cloned 2 splice variants of mouse Spata33, which they designated Spata33a and Spata33b. Full-length Spata33a encodes a deduced 132-amino acid protein with a predicted C-terminal DUF4609 domain. Spata33b contains 116 amino acids and differs from Spata33 in its C-terminal half due to a frameshift. Spata33a is serine-rich and contains several sites for serine phosphorylation and a single site for threonine phosphorylation. It also has a putative N-terminal myristoylation site, and the DUF4609 domain contains a putative N-glycosylation site. Database analysis revealed orthologs of Spata33 in mammals only. Human SPATA33 encodes a deduced 140-amino acid protein. RT-PCR of adult mouse tissues detected very high expression in testis, with much weaker expression in heart and brain, and none in kidney, liver, lung, spleen, or ovary. Spata33 expression was first detected in mouse testis soon after birth, with increased expression during the first wave of spermatogenesis. Immunohistochemical analysis of juvenile and adult mouse testis showed Spata33 uniformly distributed in the nucleus and cytosol in spermatocytes, spermatogonia, and round spermatids, but not in elongated spermatids.MAPPING
Hartz (2013) mapped the SPATA33 gene to chromosome 16q24.3 based on alignment of the SPATA33 sequence (GenBank GENBANK AK056168) with the genomic sequence (GRCh37). ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 615409 was added.
Feb. 23, 2019: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).