Endoplasmic reticulum-Golgi intermediate compartment protein 2 (ERGIC2)

The protein contains 377 amino acids for an estimated molecular weight of 42549 Da.

 

Possible role in transport between endoplasmic reticulum and Golgi. (updated: Oct. 10, 2018)

Protein identification was indicated in the following studies:

  1. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  2. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
  3. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

This protein is predicted to be membranous by TOPCONS.

Topcons

Interpro domains
Total structural coverage: 0%
Model score: 0
No model available.

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The reference OMIM entry for this protein is 612236

Endoplasmic reticulum-golgi intermediate compartment protein 2; ergic2
Cda14
Ptx1

DESCRIPTION

ERGIC2, or PTX1, is a ubiquitously expressed nuclear protein that is downregulated in prostate carcinoma (Kwok et al., 2001).

CLONING

Using subtractive hybridization, followed by RT-PCR and RACE, Kwok et al. (2001) cloned PTX1, which was expressed in normal but not cancerous prostate tissue. The predicted 377-amino acid protein has a calculated molecular mass of 42.6 kD. It contains an N-terminal nuclear localization signal, 2 N-glycosylation sites, and multiple phosphorylation sites. RT-PCR analysis detected ubiquitous expression of PTX1 in human tissues. Immunoblot analysis detected recombinant PTX1 as a 73-kD protein in both nuclear and cytoplasmic extracts of normal human prostate, with much higher levels in nuclear extracts. The high apparent molecular mass was likely due to glycosylation. Immunohistochemical analysis identified PTX1 mainly in nuclei of glandular epithelia of normal prostate and muscle cells. Nuclear expression of PTX1 was greatly reduced in prostate cancer, and the reduction was greater in advanced prostate cancer compared with early prostate cancer.

GENE FUNCTION

Liu et al. (2003) found that transfection of PTX1 into a prostate tumor cell line caused growth arrest. Introduction of antisense PTX1 into these cells resulted in uncontrolled cell growth and increased invasion potential in vitro and in nude mice.

GENE STRUCTURE

Kwok et al. (2001) determined that the PTX1 gene contains 14 exons and spans more than 40 kb.

MAPPING

By somatic cell hybrid analysis and database analysis, Kwok et al. (2001) mapped the PTX1 gene to chromosome 12p11.22. ... More on the omim web site

Subscribe to this protein entry history

Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).

Oct. 19, 2018: Protein entry updated
Automatic update: OMIM entry 612236 was added.