Casein kinase I isoform gamma-1 (CSNK1G1)
The protein contains 422 amino acids for an estimated molecular weight of 48511 Da.
Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Regulates fast synaptic transmission mediated by glutamate (By similarity). Phosphorylates CLSPN. (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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No binding partner found
The reference OMIM entry for this protein is 606274
Casein kinase i, gamma-1; csnk1g1
DESCRIPTION
Casein kinase I is the most abundant serine/threonine kinase in eukaryotic cell extracts. Multiple isoforms of the enzyme exist. The gamma-1 isoform is involved in growth and morphogenesis of eukaryotic cells (summary by Kusuda et al., 2000).CLONING
By searching an EST database for homologs of rat Csnk1g1 and by screening a testis cDNA library, Kusuda et al. (2000) obtained cDNAs encoding short and long splice variants of human CSNK1G1, which they called CSNK1G1S and CSNK1G1L, respectively. Sequence analysis predicted that the 422-amino acid CSNK1G1L protein possesses a C-terminal most terminal sequence motif (MTM), which it shares with CSNK1G2 (602214) and CSNK1G3 (604253). The CSNK1G1S protein contains 393 amino acids and is 95%, 81%, and 84% homologous to rat Csnk1g1, Csnk1g2, and Csnk1g3, respectively; it does not have the MTM. RT-PCR analysis detected ubiquitous expression of CSNK1G1L, whereas expression of CSNK1G1S was restricted to testis.MAPPING
Using FISH, Kusuda et al. (2000) mapped the CSNK1G1 gene to 15q22.1-q22.31.GENE FUNCTION
Using a protein modification screen for regulators of LRP6 (603507), Davidson et al. (2005) described the identification of Xenopus casein kinase 1-gamma (CSNK1G1), a membrane-bound member of the casein kinase-1 (CK1) family. Gain-of-function and loss-of-function experiments showed that CSNK1G1 is both necessary and sufficient to transduce LRP6 signaling in vertebrates and Drosophila cells. In Xenopus embryos, CSNK1G1 was required during anterio-posterior patterning to promote posteriorizing Wnt/beta-catenin (164820/116806) signaling. CSNK1G1 is associated with LRP6, which has multiple, modular CK1 phosphorylation sites. Wnt treatment induced the rapid CK1-gamma-mediated phosphorylation of these sites within LRP6, which, in turn, promoted the recruitment of the scaffold protein Axin (603816). Davidson et al. (2005) concluded that their results reveal an evolutionarily conserved mechanism that couples Wnt receptor activation to the cytoplasmic signal transduction apparatus.MOLECULAR GENETICS
See 606274.0001 for discussion of a possible association between variation in the CSNK1G1 gene and early infantile epileptic encephalopathy (EIEE; see 308350). ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 606274 was added.
Feb. 22, 2019: Protein entry updated
Automatic update: comparative model was added.
Feb. 22, 2019: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).