Transcription factor TFIIIB component B'' homolog (BDP1)
The protein contains 2624 amino acids for an estimated molecular weight of 293885 Da.
General activator of RNA polymerase III transcription. Requires for transcription from all three types of polymerase III promoters. Requires for transcription of genes with internal promoter elements and with promoter elements upstream of the initiation site. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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The reference OMIM entry for this protein is 607012
B-double prime 1, subunit of rna polymerase iii transcription initiation factor iiib; bdp1
Transcription factor-like nuclear regulator; tfnr
Kiaa1689
CLONING
Using a cDNA walking strategy, Kelter et al. (2000) cloned overlapping partial sequences of TFNR from fetal brain and spinal cord libraries. The deduced 2,254-amino acid protein has a calculated molecular mass of 250 kD. All cDNAs isolated from one fetal brain cDNA library lacked exon 15 resulting in a truncated protein of 796 amino acids. The TFNR protein is highly hydrophilic. It contains a 55-amino acid motif that is repeated as 6 highly conserved and 3 less well-conserved copies encoded by exon 17. It also contains a bipartite nuclear localization signal, a DNA-ligase A1 ATP-dependent (126391) binding site, and a second bipartite nuclear localization signal in the C terminus. Sequence comparison with the first 11 exons of mouse Tfnr, encoding 545 amino acids, indicated 77% identity to the human TFNR protein. A stretch of over 300 amino acids of human TFNR showed 29% identity with the yeast TFC5 protein, a component of the TFIIIB transcription initiation complex. Northern blot analysis detected weak expression of 9.5-, 3.1-, and 1.6-kb transcripts in all tissues examined, and a 1.9-kb transcript expressed only in brain. Analysis of mRNAs from several brain areas showed a strong signal at 9.5 and 1.9 kb in cerebellum, and a weak signal in all other brain areas. Western blot analysis of fetal and adult human tissues showed 5 bands ranging from 55 to 250 kD. Immunofluorescent localization of TFNR in HeLa cells revealed a punctate nuclear staining pattern. Schramm et al. (2000) also cloned BDP1, which they called hB-double prime, and found that the protein has several potential phosphorylation sites. Schramm et al. (2000) isolated 2 variants, one of which is likely to be a splice variant. The other variant contains an insertion that results in the replacement of the last 21 amino acids with 5 different amino acids. Western blot analysis of HeLa cells showed BDP1 bands at about 160 and 250 kD.GENE STRUCTURE
Kelter et al. (2000) determined that the TFNR gene contains 32 exons and spans about 80 kb.GENE FUNCTION
With use of anti-BDP1 antibodies in in vitro transcription reactions, Schramm et al. (2000) determined that BPD1 is required for transcription from the Ad2 VAI and human U6 (180692) RNA polymerase III promoters. By chromatin immunoprecipitation assays, they also found that BDP1 binds the U6 promoter region in vivo, further suggesting that BDP1 is part of the U6 initiation complex.MAPPING
By FISH, Kelter et al. (2000) mapped the TFNR gene to chromosome 5q13. Further analysis of this region indicated that TFNR lies just outside the repeated SMA1 (253300) region. Sequencing of a mouse BAC clone revealed a head-to-head orientation between the Tfnr and Serf1 (603011) genes. ... More on the omim web site
Nov. 17, 2018: Protein entry updated
Automatic update: OMIM entry 607012 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).