Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform (PPP2CB)
The protein contains 309 amino acids for an estimated molecular weight of 35575 Da.
PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Apoptotic mitochondrial changes
Negative regulation of Ras protein signal transduction
Peptidyl-serine dephosphorylation
Peptidyl-threonine dephosphorylation
Positive regulation of microtubule binding
Proteasome-mediated ubiquitin-dependent protein catabolic process
Protein dephosphorylation
Regulation of gene expression
Response to antibiotic
Response to endoplasmic reticulum stress
Response to hydrogen peroxide
Response to lead ion
Cellular Component
Chromosome, centromeric region
Cytosol
Nucleus
Protein phosphatase type 2A complex
Spindle pole
The reference OMIM entry for this protein is 176916
Protein phosphatase 2, catalytic subunit, beta isoform; ppp2cb
Protein phosphatase 2a, catalytic subunit, beta isoform
Pp2cb
CLONING
Hemmings et al. (1988) isolated the human cDNA for the beta-isoform of the catalytic subunit of protein phosphatase 2A (PPP2CB) from lung and lung fibroblast libraries. The cDNA encodes a 309-amino acid polypeptide.GENE FUNCTION
Using affinity chromatography and mass spectrometric analysis, Hoffmeister et al. (2014) found that mouse Nosip (616759) interacted with a PP2A complex containing the catalytic subunits Pp2aca (PPP2CA; 176915) and Pp2acb, the scaffolding subunit Pr65-alpha (PPP2R1A; 605983), and the regulatory subunit Pr55-alpha (PPP2R2B; 604325). Protein pull-down experiments confirmed that Nosip precipitated the PP2A holoenzyme. An in vitro ubiquitination assay showed that Nosip functioned as an E3 ubiquitin ligase in monoubiquitination of the catalytic PP2A subunit. Monoubiquitination did not cause proteasomal degradation of PP2A, but it appeared to downregulate PP2A activity via a nondegradative mechanism. Loss of Nosip in mice resulted in increased PP2A activity in craniofacial tissue during development, resulting in holoprosencephaly and facial anomalies. Hoffmeister et al. (2014) concluded that NOSIP modulates PP2A activity in craniofacial development.MAPPING
Jones et al. (1993) mapped the PPP2CB gene to chromosome 8 by use of the polymerase chain reaction in the study of somatic cell hybrids. By fluorescence in situ hybridization, they further localized the PPP2CB gene to 8p12-p11.2. Southern blot analysis of somatic cell hybrid DNA suggested that a pseudogene of the PPP2CB catalytic subunit is on chromosome 16. Imbert et al. (1996) constructed a YAC contig map of the 8p21-p12 chromosomal region and precisely mapped the PPP2CB within the YAC contigs.NOMENCLATURE
The symbol PP2CB was used at one time for the gene now symbolized PPM1B (603770), which is located on 2p21. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 176916 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).