Vacuolar protein sorting-associated protein 37C (VPS37C)
The protein contains 355 amino acids for an estimated molecular weight of 38659 Da.
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation. (updated: Feb. 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs2232142 | |
| dbSNP:rs754382 | |
| dbSNP:rs4297482 |
Biological Process
Endosomal transport
Endosome transport via multivesicular body sorting pathway
Intracellular transport of virus
Macroautophagy
Membrane organization
Multivesicular body assembly
Protein targeting to membrane
Protein targeting to vacuole
Protein transport
Ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
Viral budding via host ESCRT complex
Viral life cycle
Viral process
Viral protein processing
Virion assembly
The reference OMIM entry for this protein is 610038
Vacuolar protein sorting 37, yeast, homolog of, c; vps37c
DESCRIPTION
VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I), a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al., 2005).CLONING
By searching a database for sequences homologous to yeast Vps37, Stuchell et al. (2004) identified human VPS37C. The deduced 523-amino acid protein contains a central mod(r) (modifier of rudimentary) domain and 2 coiled-coil regions, one overlapping the N-terminal border of the mod(r) domain and the other within the mod(r) domain. Using TSG101 (601387) as bait in a yeast 2-hybrid screen of a peripheral mononuclear lymphocyte cDNA library, Eastman et al. (2005) cloned VPS37C. The deduced 355-amino acid protein contains an N-terminal mod(r) domain and a C-terminal proline-rich domain.GENE FUNCTION
Eastman et al. (2005) found that VPS37C formed a ternary complex with TSG101 and VPS28 (601387) by binding a C-terminal domain of TSG101. VPS37C also bound HRS (HGS; 604375), another class E VPS factor. VPS37C was recruited to aberrant endosomes induced by overexpression of TSG101, HRS, or a dominant-negative form of VPS4 (VPS4A; 609982). Enveloped viruses that encode PTAP motifs to facilitate budding, like human immunodeficiency virus (HIV)-1 Gag protein, exploit ESCRT-I as an interface with the class E VPS pathway. VPS37C was recruited to the plasma membrane along with TSG101 by HIV-1 Gag. Moreover, direct fusion of VPS37C to HIV-1 Gag obviated the requirement for a PTAP motif to induce virion release. Depletion of VPS37C from human embryonic kidney cells did not inhibit murine leukemia virus budding, which is not mediated by ESCRT-I. However, if murine leukemia virus budding was engineered to be ESCRT-I-dependent, it was inhibited by VPS37C depletion, and the inhibition was accentuated if VPS37B (610037) was simultaneously depleted. Eastman et al. (2005) concluded that VPS37C is a functional component of mammalian ESCRT-I.MAPPING
The International Radiation Hybrid Mapping Consortium mapped the VPS37C gene to chromosome 11 (TMAP SHGC-31731). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 610038 was added.
Feb. 25, 2016: Protein entry updated
Automatic update: model status changed
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed
Jan. 25, 2016: Protein entry updated
Automatic update: model status changed