cAMP-dependent protein kinase catalytic subunit beta (PRKACB)
The protein contains 351 amino acids for an estimated molecular weight of 40623 Da.
Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (PubMed:12420224, PubMed:21423175). Phosphorylates GPKOW which regulates its ability to bind RNA (PubMed:21880142). (updated: Jan. 31, 2018)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology, is annotated as membranous in UniProt.
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| Variant | Description |
|---|---|
| dbSNP:rs36117118 |
Biological Process
Activation of phospholipase C activity
Activation of protein kinase A activity
Adaptive immune response
Adenylate cyclase-modulating G protein-coupled receptor signaling pathway
Blood coagulation
Carbohydrate metabolic process
Cellular response to glucagon stimulus
Chemical synaptic transmission
Energy reserve metabolic process
Epidermal growth factor receptor signaling pathway
Fibroblast growth factor receptor signaling pathway
Gluconeogenesis
Glucose metabolic process
High-density lipoprotein particle assembly
Innate immune response
Intracellular signal transduction
Negative regulation of inflammatory response to antigenic stimulus
Negative regulation of meiotic cell cycle
Negative regulation of smoothened signaling pathway involved in dorsal/ventral neural tube patterning
Neural tube closure
Neurotrophin TRK receptor signaling pathway
Pathogenesis
Peptidyl-serine phosphorylation
Protein kinase A signaling
Protein phosphorylation
Regulation of insulin secretion
Regulation of protein processing
Renal water homeostasis
Response to clozapine
Signal transduction
Small molecule metabolic process
Stimulatory C-type lectin receptor signaling pathway
Transmembrane transport
Triglyceride catabolic process
Water transport
Cellular Component
CAMP-dependent protein kinase complex
Centrosome
Ciliary base
Cytosol
Extracellular exosome
Intercellular bridge
Nucleoplasm
Nucleus
Perinuclear region of cytoplasm
Plasma membrane
Synapse
Molecular Function
AMP-activated protein kinase activity
ATP binding
CAMP-dependent protein kinase activity
Kinase activity
Magnesium ion binding
Protein kinase A regulatory subunit binding
Protein serine kinase activity
Protein serine/threonine kinase activity
Protein threonine kinase activity
Ubiquitin protein ligase binding
The reference OMIM entry for this protein is 176892
Protein kinase, camp-dependent, catalytic, beta; prkacb
One of the best characterized classes of kinases is the cAMP-dependent protein kinases (PKA). Two PKA isoforms exist, designated types I and II, which differ in their dimeric R subunits, designated RI and RII, respectively. Furthermore, there are at least 2 products for each of the RI and RII subunits: RI-alpha (188830), RI-beta (176911), RII-alpha (176910), and RII-beta (176912). The C subunit has also been shown to consist of more than one gene product. Mammalian C-alpha, C-beta, and C-gamma cDNAs have been cloned. Berube et al. (1991) assigned the catalytic subunit C-beta of PKA to human chromosome 1 by Southern blot analysis of somatic cell hybrids. By in situ hybridization, the gene was localized to 1p36.1. (Also see Simard et al. (1992).) ... More on the omim web site
Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 25, 2017: Additional information
No protein expression data in P. Mayeux work for PRKACB
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 176892 was added.