Dynactin subunit 6 (DCTN6)
The protein contains 190 amino acids for an estimated molecular weight of 20747 Da.
No function (updated: Jan. 7, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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The reference OMIM entry for this protein is 612963
Dynactin 6; dctn6
Ws3
P27
DESCRIPTION
Dynactin (see 601143) is a multimeric protein essential for minus-end-directed transport driven by the microtubule-based motor dynein (see DYNC1H1; 600112). DCTN6 is a subunit of the pointed-end subcomplex of dynactin that is thought to interact with membranous cargo (Parisi et al., 2004).CLONING
By searching for genes in a region of chromosome 8 associated with Werner syndrome (277700), followed by TAIL-PCR and 5-prime and 3-prime RACE, Ichikawa et al. (1997) cloned DCTN6, which they called WS3. The deduced 190-amino acid protein has a calculated molecular mass of 20.7 kD. It has several hydrophobic stretches, 4 potential phosphorylation sites, 3 potential myristoylation sites, and an RGD motif that may mediate cell attachment. Northern blot analysis detected a DCTN6 transcript of about 1.0 kb in all tissues examined. Zoo blot analysis detected putative orthologs in other species, including yeast. By searching databases for proteins containing an isoleucine-patch motif predicted to adopt a left-handed parallel beta-helix fold, Parisi et al. (2004) identified human DCTN6, which they called p27.GENE STRUCTURE
Ichikawa et al. (1997) determined that the DCTN6 gene contains 7 exons.MAPPING
Using FISH and mouse-human hybrid cell lines, Ichikawa et al. (1997) mapped the DCTN6 gene to chromosome 8p12-p11, telomeric to the RBPMS gene (601558). Southern blot analysis detected a single DCTN6 gene. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 612963 was added.
Jan. 28, 2016: Protein entry updated
Automatic update: model status changed
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed