Tyrosine-protein phosphatase non-receptor type 7 (PTPN7)
The protein contains 360 amino acids for an estimated molecular weight of 40529 Da.
Protein phosphatase that acts preferentially on tyrosine-phosphorylated MAPK1. Plays a role in the regulation of T and B-lymphocyte development and signal transduction. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
(right-click above to access to more options from the contextual menu)
Biological Process
Cellular response to cytokine stimulus
MAPK cascade
Peptidyl-tyrosine dephosphorylation
Protein dephosphorylation
The reference OMIM entry for this protein is 176889
Protein-tyrosine phosphatase, nonreceptor-type, 7; ptpn7
Protein-tyrosine phosphatase, nonreceptor-type, stress-induced; ptpni
CLONING
Adachi et al. (1992) identified a protein-tyrosine phosphatase expressed in pre-B cells by reverse transcriptase-polymerase chain reaction technique. Adachi et al. (1992) reported the sequence of the full-length cDNA of this leukocyte PTP (PTPN7) and showed that the mRNA was predominantly expressed in hematopoietic cells. Leukocyte PTP, which Adachi et al. (1992) referred to as LC-PTP, showed 53.8% nucleotide sequence similarity in the catalytic domains to leukocyte-common antigen (LCA; 151460).MAPPING
By fluorescence in situ hybridization, Adachi et al. (1992) mapped the PTPN7 gene 1q32.1 where chromosomal deletions are observed in non-Hodgkin lymphomas. Because PTPN7 and LCA are predominantly expressed in hematopoietic cells and both genes map to 1q32, Adachi et al. (1992) suggested that the 2 genes probably arose from a common ancestral gene and the transcriptional machinery of the 2 genes may be similarly regulated. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
June 20, 2017: Protein entry updated
Automatic update: comparative model was added.
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 176889 was added.
Jan. 28, 2016: Protein entry updated
Automatic update: model status changed
Jan. 25, 2016: Protein entry updated
Automatic update: model status changed