40S ribosomal protein S7 (RPS7)

The protein contains 194 amino acids for an estimated molecular weight of 22127 Da.

 

Required for rRNA maturation. (updated: March 4, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  5. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
  6. Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

Interpro domains
Total structural coverage: 100%
Model score: 100
No model available.

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The reference OMIM entry for this protein is 603658

Ribosomal protein s7; rps7

DESCRIPTION

The eukaryotic ribosome consists of more than 80 different ribosomal proteins, including RPS7, and 4 RNA species (see 180450).

CLONING

Annilo et al. (1995) isolated cDNAs encoding RPS7 by screening a human cDNA expression library with antibodies against rat Rps7. The deduced 194-amino acid human RPS7 protein is identical to rat Rps7. Northern blot analysis detected an approximately 0.7-kb RPS7 transcript in HeLa cells. The authors identified several RPS7 pseudogenes.

GENE STRUCTURE

Annilo et al. (1995) determined that the RPS7 gene contains 7 exons and spans over 6 kb.

MAPPING

By FISH, Annilo et al. (1995) mapped the RPS7 gene to chromosome 2p25. Kenmochi et al. (1998) confirmed the mapping assignment reported by Annilo et al. (1995).

MOLECULAR GENETICS

Gazda et al. (2008) screened 196 probands with Diamond-Blackfan anemia (see DBA8, 612563) for mutations in 25 genes encoding ribosomal proteins and identified a splice site mutation in the RPS7 gene in 1 proband (603658.0001). The mutation was not found in his unaffected sister or at least 150 controls, and functional studies strongly suggested that mutation in RPS7 is associated with defects in the maturation of ribosomal RNAs. ... More on the omim web site

Subscribe to this protein entry history

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

March 15, 2016: Protein entry updated
Automatic update: OMIM entry 603658 was added.