Alexandre G. de Brevern's publications

Year 2013

  1. Rebhemed J, Alphand V, de Berardinis V, de Brevern A.G.#
    Evolution study of the Baeyer-Villiger monooxygenases enzyme family: functional importance of the highly conserved residues
    Biochimie (2013) 95(7):1394-402.

    aeyer-Villiger monooxygenases (BVMOs) catalyze the transformation of linear and cyclic ketones into their corresponding esters and lactones by introducing an oxygen atom into a C-C bond. This bioreaction has numerous advantages compared to its chemical version; it does not induce the use of potentially harmful reagents (i.e., green chemistry) and displays significant better enantio- and regio-selectivity. New potential BVMOs were searched using sequence homology for type I BVMO proteins. 116 new sequences were identified as new putative BVMOs respecting the defined selection criteria. Multiple sequence alignments were carried out on the selected sequences to study the conservation of structurally and/or functionally important amino acids during evolution. Type I BVMO signature motif was found to be conserved in 94.8% of the sequences. We noticed also the highly conserved - but previously unnoticed - Threonine 167 (93.1%), located in the signature motif; this position could be added in the pattern used to characterize specific Type I enzymes. Amino acids at the vicinity of the FAD and NADPH cofactors were found also to be highly conserved and the details of the interactions were emphasized. Interestingly, residues at the enzyme binding site were found less conserved in terms of sequence evolution, leading sometimes to some important amino acid changes. These behaviors could explain the enzyme selectivity and specificity for different ligands.
    preprint paper .
    Supplementary_Material Supplementary_Material

    collaboration with Dr. Véronique Alphand of Institute of Molecular Science (CNRS, Univ Aix Marseille, Marseille, France) and Dr. Véronique de Berardinis Genoscope (CEA, Evry, France).

    • The Ministry of Research (France)
    • The University Paris Diderot, Sorbonne Paris Cite (France)
    • The National Institute of Blood Transfusion (INTS, France)
    • The National Institute of Health and Medical Research (INSERM, France)
    • The Laboratories of Excellence, GR-Ex (France)
    • ANR Naturadyre [Projet ANR- 10-CD2I-014] - Réactions d’oxydation biocatalysées pour la synthèse de composés naturels ou énantiopurs (France)
    • The National Center for Atomic Research (CEA,France)
    • University d’Aix-Marseille (France)
    • The National Center for Scientific Research (CNRS,France)
    • high performance computing (HPC) resources at the French National Computing Center CINES under grant no. 2012-c2012076930 funded by the GENCI (Grand Equipement National de Calcul Intensif).

Alexandre G. de Brevern
Last Modification : January 2014
Paris7 Inserm INTS INTS GR-Ex Sorbonne Paris Cite