60S ribosomal protein L7a (RPL7A)

The protein contains 266 amino acids for an estimated molecular weight of 29996 Da.

 

No function (updated: April 1, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  5. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
  6. Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

Interpro domains
Total structural coverage: 100%
Model score: 100
MODL_I-TASSER-3.0

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VariantDescription
dbSNP:rs12295

The reference OMIM entry for this protein is 185640

Ribosomal protein l7a; rpl7a
Surfeit 3; surf3

The mammalian ribosome is composed of 4 RNA species (see 180450) and approximately 80 different proteins (see 180466).

CLONING

Giallongo et al. (1989) demonstrated that the SURF3 gene encodes the L7a ribosomal protein. The first 2 exons of the human SURF3 gene have been found to be capable of activating the TRK oncogene (see 191315). The SURF3 gene appears to be the only intron-containing member of the surfeit family cluster on chromosome 9q34 (see SURF1, 185620).

GENE STRUCTURE

Angiolillo et al. (2002) determined that transcription start sites of the MED22 (185641) and RPL7A genes are separated by a 110-bp region that shows bidirectional promoter activity. This promoter region lacks a TATA box but is characterized by a CpG island that extends through the first exon into the first intron of both genes.

MAPPING

Yon et al. (1993) mapped the RPLA7 gene to chromosome 9q34.1. Kenmochi et al. (1998) confirmed the mapping assignment.

MOLECULAR GENETICS

Mor et al. (1996) described a common polymorphism in the surfeit gene cluster that was of interest because it occurs in the TSC1 (191100) region of chromosome 9q34. By database analysis, Smith (1997) found that the polymorphism reported by Mor et al. (1996) is due to mutation in the SURF3 gene. ... More on the omim web site

Subscribe to this protein entry history

May 12, 2019: Protein entry updated
Automatic update: model status changed

Nov. 16, 2018: Protein entry updated
Automatic update: model status changed

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

Oct. 26, 2017: Protein entry updated
Automatic update: model status changed

March 15, 2016: Protein entry updated
Automatic update: OMIM entry 185640 was added.

Jan. 24, 2016: Protein entry updated
Automatic update: model status changed