Microtubule-associated protein 1S (MAP1S)
The protein contains 1059 amino acids for an estimated molecular weight of 112211 Da.
Microtubule-associated protein that mediates aggregation of mitochondria resulting in cell death and genomic destruction (MAGD). Plays a role in anchoring the microtubule organizing center to the centrosomes. Binds to DNA. Plays a role in apoptosis. Involved in the formation of microtubule bundles (By similarity). (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs17710707 | |
| dbSNP:rs17710707 | |
| dbSNP:rs7252905 |
Biological Process
Apoptotic DNA fragmentation
Apoptotic process
Autophagy
Axonogenesis
Brain development
Dendrite development
DNA metabolic process
Execution phase of apoptosis
Microtubule bundle formation
Microtubule cytoskeleton organization
Mitochondrion transport along microtubule
Nervous system development
Neuron projection morphogenesis
Regulation of chromatin disassembly
Regulation of microtubule depolymerization
The reference OMIM entry for this protein is 607573
Microtubule-associated protein 1s; map1s
Map8
Bpy2-interacting protein 1; bpy2ip1
Vcy2-interacting protein 1; vcy2ip1
Chromosome 19 open reading frame 5; c19orf5
CLONING
Using the SEC1 homology domain of LRPPRC (607544) as probe in a yeast 2-hybrid screen, Liu and McKeehan (2002) cloned VCY2IP1 (BPY2IP1), which they called C19ORF5, from a liver cDNA library. The deduced 672-amino acid protein is proline-rich (12.8%) and contains an arginine-rich potential RNA-binding motif. VCY2IP1 shares significant homology with several microtubule-associated proteins. Northern blot analysis revealed a major transcript of about 3 kb in all 12 tissues tested. Additional less-abundant transcripts were detected in some tissues.GENE FUNCTION
Wang et al. (2012) found that epitope-tagged human nemitin (WDR47; 615734) was expressed diffusely in the cytoplasm in transfected COS-7 cells. Coexpression with mouse Map8 resulted in redistribution of nemitin to the microtubule network. Domain analysis revealed that the C-terminal WD40 domain of nemitin interacted with the N-terminal domain of proteolytically processed Map8 light chain.MAPPING
Gross (2014) mapped the MAP1S gene to chromosome 19p13.11 based on an alignment of the MAP1S sequence (GenBank GENBANK BC080547) with the genomic sequence (GRCh37). ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 607573 was added.