Microsomal glutathione S-transferase 3 (MGST3)
The protein contains 152 amino acids for an estimated molecular weight of 16516 Da.
Catalyzes oxydation of hydroxy-fatty acids (PubMed:9278457). Also catalyzes the conjugation of a reduced glutathione to leukotriene A4 in vitro (PubMed:9278457). May participate in the lipid metabolism (PubMed:9278457). (updated: June 17, 2020)
Protein identification was indicated in the following studies:
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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| Variant | Description |
|---|---|
| dbSNP:rs1802087 | |
| dbSNP:rs1802088 |
Biological Process
Aflatoxin metabolic process
Glutathione derivative biosynthetic process
Leukotriene biosynthetic process
Lipid metabolic process
Response to organonitrogen compound
Xenobiotic metabolic process
The reference OMIM entry for this protein is 604564
Glutathione s-transferase, microsomal, 3; mgst3
CLONING
5-Lipoxygenase-activating protein (FLAP; 603700), leukotriene C4 synthase (LTC4S; 246530), and microsomal glutathione S-transferase-2 (MGST2; 601733) are members of a family of small, membrane-associated proteins. By searching a sequence database using the MGST2 protein sequence as query, Jakobsson et al. (1997) identified an EST encoding MGST3. The deduced 152-amino acid MGST3 protein has a calculated molecular mass of 16.5 kD and a calculated isoelectric point of 10.2. Like FLAP, LTC4S, and MGST2, MGST3 contains 3 predicted hydrophobic regions separated by hydrophilic domains. MGST3 shares 36% amino acid sequence identity with MGST2, 27% identity with LTC4S, 22% identity with MGST1 (138330), and 20% identity with FLAP. Northern blot analysis detected a 0.6-kb MGAT3 transcript in a wide variety of human tissues, with the highest expression in heart, skeletal muscle, and adrenal cortex.GENE FUNCTION
In in vitro assays, recombinant MGST3 showed glutathione-dependent peroxidase activity and LTC4S activity (Jakobsson et al., 1997).MAPPING
By FISH, Jakobsson et al. (1997) mapped the MGST3 gene to chromosome 1q23.MOLECULAR GENETICS
- Exclusion Studies Thameem et al. (2003) genotyped unique representative SNPs in the MGST3 gene in selected diabetic and nondiabetic Pima Indians and found no evidence of association with type II diabetes mellitus (125853). Furthermore, interindividual variation of skeletal muscle MGST3 mRNA was not correlated with differences in insulin (176730) action in nondiabetic subjects. Thameem et al. (2003) concluded that alterations of MGST3 are unlikely to contribute to type II diabetes mellitus or differences in insulin sensitivity in the Pima Indians. ... More on the omim web site
June 29, 2020: Protein entry updated
Automatic update: Entry updated from uniprot information.
May 11, 2019: Protein entry updated
Automatic update: Entry updated from uniprot information.
Dec. 10, 2018: Protein entry updated
Automatic update: model status changed
Nov. 17, 2018: Protein entry updated
Automatic update: OMIM entry 604564 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).