TATA-binding protein-associated factor 2N (TAF15)
The protein contains 592 amino acids for an estimated molecular weight of 61830 Da.
RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
MRNA stabilization
Positive regulation of transcription, DNA-templated
Regulation of signal transduction by p53 class mediator
RNA splicing
Transcription by RNA polymerase II
Transcription elongation from RNA polymerase II promoter
Transcription initiation from RNA polymerase II promoter
The reference OMIM entry for this protein is 601574
Taf15 rna polymerase ii, tata box-binding protein-associated factor, 68-kd; taf15
Tata box-binding protein-associated factor 2n; taf2n
Tbp-associated factor, rna polymerase ii, 68-kd
Rna-binding protein 56; rbp56 rbp56/nr4a3 fusion gene, includ
CLONING
The EWS gene (EWSR1; 133450) was found at the chromosome breakpoints in Ewing sarcoma (612219) and maps to chromosome 22; the FUS/TLS gene (137070) was found at the breakpoints of myxoid liposarcoma and acute myeloid leukemia and maps to chromosome 16. These genes encode proteins that carry a highly homologous RNA binding domain. Fusion proteins made between the N-terminal half of either gene and transcriptional regulatory proteins, also derived from genes located at breakpoints, appear to be involved in the pathogenesis of tumors. By PCR amplification of human Namalwa cell cDNA using degenerate primers made from the conserved amino acid sequences in the RNA binding domain of EWS and FUS/TLS, Morohoshi et al. (1996) obtained a cDNA fragment, designated RBP56. This fragment was predicted to encode an amino acid sequence similar but not identical to those of EWS and FUS/TLS. Using this fragment as a probe, they obtained 2 isoforms of cDNAs consisting of 2,144 and 2,153 bp, respectively, which encoded proteins consisting of 589 and 592 amino acid residues, respectively. The expression of RBP56 mRNA was observed in all human fetal and adult tissues examined, as was the expression of EWS and FUS/TLS mRNAs.GENE STRUCTURE
By genomic sequence analysis, Morohoshi et al. (1998) determined that the RBP56 gene spans about 37 kb and contains 16 exons ranging in size from 33 bp to 562 bp. The longest, exon 15, encodes a C-terminal region with 19 repeats. The authors noted that the conservation of the overall exon number and structure of RBP56, FUS/TLS, and EWS indicates that they probably originated from the same ancestor gene.MAPPING
Morohoshi et al. (1996) mapped the TAF15 (RBP56) gene to chromosome 17 by Southern blot analysis of mouse/human somatic cell hybrids and localized it to 17q11.2-q12 by FISH. By PCR analysis using genomic DNA from human/rodent somatic cell hybrid panels harboring part of human chromosome 17, they further localized the gene to 17q11.1-q11.2.CYTOGENETICS
- RBP56/NR4A3 Fusion Gene Although most extraskeletal myxoid chondrosarcomas (EMC; 612237) are characterized by the translocation t(9;22)(q22;q12), another subset carries a t(9;17)(q22;q11). The t(9;22) is known to result in fusion of the EWS gene on 22q12 with the NR4A3 gene (600542) on 9q11, creating a chimeric EWS/NR4A3 gene. Panagopoulos et al. (1999) examined 2 extraskeletal myxoid chondrosarcomas with t(9;17)(q22;q11) and found that the NR4A3 gene, which they designated CHN, was combined with the RBP56 gene from 17q11 to generate a chimeric RBP56/NR4A3 gene. RBP56 had not previously been implicated in tumorigenesis, but it encodes a putative RNA-binding protein similar to the EWS and FUS genes known to play a pathogenetic role in sarcomas. The presence of the RBP56/NR4A3 chimeric gene in extraskeletal myxoid chondrosarcomas showed that the N-terminal parts of EWS and RBP56 have similar oncogenic potential, making them pathogenetically equivalent in oncoproteins arising from fusions with certain transcription factors. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 601574 was added.