Sorting nexin-15 (SNX15)
The protein contains 342 amino acids for an estimated molecular weight of 38291 Da.
May be involved in several stages of intracellular trafficking. Overexpression of SNX15 disrupts the normal trafficking of proteins from the plasma membrane to recycling endosomes or the TGN. (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
(right-click above to access to more options from the contextual menu)
| Variant | Description |
|---|---|
| dbSNP:rs495820 |
The reference OMIM entry for this protein is 605964
Sorting nexin 15; snx15
SNX15 is a member of the sorting nexin family of phox homology domain (PX)-containing proteins that are homologous to yeast proteins involved in protein trafficking (Phillips et al., 2001).
CLONING
By EST database searching with a consensus sequence for the PX domain of several SNX molecules, followed by nested PCR on brain, lung, and liver cDNA libraries, Phillips et al. (2001) obtained a full-length SNX15 cDNA. SNX15 encodes a deduced 342-amino acid protein as well as a 256-amino acid splice variant, designated SNX15A. SNX15 contains a 124-amino acid PX domain in the N terminus and a novel 73-amino acid domain in the C terminus, which the authors designated the ESP domain. SNX15A is identical to SNX15 except that it lacks 86 amino acids within its C terminus, including a large portion of the ESP domain. Northern blot analysis detected ubiquitous expression of an approximately 2-kb SNX15 transcript, with highest expression in skeletal muscle, heart, brain, kidney, spleen, thymus, and small intestine. Screening of 8 tissues by PCR detected SNX15 expression in lung, liver, skeletal muscle, prostate, pancreas, and adult and fetal brain, and SNX15A expression in adult brain, liver, and placenta. Although predicted to be a soluble protein, both endogenous and overexpressed SNX15 were found on membranes and in the cytosol.MAPPING
Phillips et al. (2001) determined that SNX15 is identical to a clone identified as an expressed gene on chromosome 11q13 by Guru et al. (1997).GENE FUNCTION
Using cotransfection experiments, Phillips et al. (2001) showed that SNX15 associates with itself as well as with SNX1 (601272), SNX2 (605929), SNX4 (605931), and platelet-derived growth factor receptor (PDGFR; see 173490). Its PX domain is required for its membrane association and for its association with PDGFR. No association of SNX15 with receptors for epidermal growth factor (131550) or insulin (147670) was found, but overexpression of SNX15 led to a decrease in the processing of insulin and hepatocyte growth factor receptors (164860) to their mature subunits. Immunofluorescence studies showed that overexpression of SNX15 resulted in mislocalization of furin (136950), the endoprotease responsible for cleavage of insulin and hepatocyte growth factor receptors. ... More on the omim web site
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 605964 was added.