Protein NipSnap homolog 2 (GBAS)
The protein contains 286 amino acids for an estimated molecular weight of 33743 Da.
May act as a positive regulator of L-type calcium channels. (updated: Nov. 22, 2017)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
ATP biosynthetic process
Mitochondrion organization
Negative regulation of ATP citrate synthase activity
Oxidative phosphorylation
Positive regulation of high voltage-gated calcium channel activity
Cellular Component
Integral component of plasma membrane
Membrane
Mitochondrial outer membrane
Mitochondrion
Molecular Function
The reference OMIM entry for this protein is 603004
Glioblastoma amplified sequence; gbas
4-@nitrophenylphosphatase domain and nonneuronal snap25-like 2; nipsnap2
Nipsnap, c. elegans, homolog of, 2
CLONING
Chromosomal region 7p12, which contains the EGFR (131550) gene, is amplified in approximately 40% of glioblastomas, the most common and malignant form of central nervous system tumor. By screening DNA from glioblastomas with ESTs from this region, Wang et al. (1998) identified a gene that was amplified in 2 of 12 tumors and designated it GBAS (glioblastoma amplified sequence). The predicted 286-amino acid protein contains a signal peptide, a transmembrane domain, and 2 tyrosine phosphorylation sites. Northern blot analysis revealed that the 2.2-kb GBAS transcript is expressed in various tissues, most abundantly in heart and skeletal muscle. Seroussi et al. (1998) identified GBAS as a member of the NIPSNAP gene family and designated it NIPSNAP2. See NIPSNAP1 (603249). They also identified cDNAs encoding mouse Nipsnap2. The predicted mouse and human NIPSNAP2 proteins are 91% identical.MAPPING
By use of a radiation hybrid panel, Wang et al. (1998) mapped the GBAS gene to within approximately 880 kb of EGFR at 7p12. ... More on the omim web site
Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 603004 was added.