NSFL1 cofactor p47 (NSFL1C)
The protein contains 370 amino acids for an estimated molecular weight of 40573 Da.
Reduces the ATPase activity of VCP (By similarity). Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis (By similarity). May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro) (PubMed:15498563). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (PubMed:23649807). Also, regulates spindle orientation during mitosis (PubMed:23649807). (updated: May 23, 2018)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is annotated as membranous in Gene Ontology.
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| Variant | Description |
|---|---|
| dbSNP:rs9575 |
Biological Process
Autophagosome assembly
Establishment of mitotic spindle orientation
Golgi organization
Membrane fusion
Negative regulation of protein localization to centrosome
Nuclear membrane reassembly
Positive regulation of mitotic centrosome separation
Proteasome-mediated ubiquitin-dependent protein catabolic process
Regulation of catalytic activity
The reference OMIM entry for this protein is 606610
Nsfl1 cofactor; nsfl1c
P47
CLONING
The ATPases NSF (601633) and p97 (VCP; 601023) are involved in the heterotypic fusion of transport vesicles with their target membranes and the homotypic fusion of membrane compartments. By biochemical purification of a 47-kD protein complexed with p97 in rat liver cytosol, followed by micropeptide sequence analysis and PCR with degenerate primers, Kondo et al. (1997) isolated a cDNA encoding rat p47. Rat p47 binds specifically to p97. Northern blot analysis revealed ubiquitous expression of a 1.5-kb p47 transcript in rat tissues. Kondo et al. (1997) also identified a human EST with approximately 89% sequence identity to rat p47. They concluded that the p97/p47 complex is probably the active component in the fusion reaction that leads to cisternal regrowth. Zhang et al. (2000) screened CD34 (142230)-positive hemopoietic stem/progenitor cells for novel cDNAs and obtained a human homolog of p47. The deduced human p47 protein has 370 amino acids. Northern blot and macroarray analyses revealed abundant and ubiquitous expression of p47.GENE STRUCTURE
Zhang et al. (2000) determined that human p47 is encoded by a single exon.MAPPING
Zhang et al. (2000) mapped the p47 gene to the distal end of chromosome 20p by STS analysis. ... More on the omim web site
May 26, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 606610 was added.
Feb. 24, 2016: Protein entry updated
Automatic update: model status changed