Secretory carrier-associated membrane protein 3 (SCAMP3)
The protein contains 347 amino acids for an estimated molecular weight of 38287 Da.
Functions in post-Golgi recycling pathways. Acts as a recycling carrier to the cell surface. (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
This protein is predicted to be membranous by TOPCONS.
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| Variant | Description |
|---|---|
| dbSNP:rs760073 | |
| dbSNP:rs1318328 | |
| dbSNP:rs909106 | |
| dbSNP:rs909107 |
The reference OMIM entry for this protein is 606913
Secretory carrier membrane protein 3; scamp3
Propin1
CLONING
Singleton et al. (1997) obtained partial SCAMP3 cDNA clones from a HeLa cell cDNA library by hybridization with an EST showing homology to SCAMP1 (606911). They used restriction digestion and PCR amplification to clone the full-length cDNA. SCAMP3 encodes a deduced 347-amino acid protein with a calculated molecular mass of 38.3 kD. The protein shares structural features with SCAMP1 and SCAMP2 (606912), including a leucine zipper-like segment, a proline-rich element, an extended central core that includes 4 putative transmembrane domains, a polar segment, and an alanine-rich C terminus. SCAMP3 shares 57% and 56% overall sequence identity with SCAMP1 and SCAMP2, respectively, and 89% identity with the mouse homolog. The most divergent regions are in the N terminus. Northern blot analysis detected a 1.4-kb SCAMP3 transcript, with highest expression in heart and skeletal muscle, intermediate expression in placenta and pancreas, and low expression in brain, lung, liver, and kidney. Immunofluorescent localization in HeLa cells showed punctate staining enriched in the cell periphery, with some colocalization with SCAMP1 and SCAMP2. Winfield et al. (1997) found comparable expression of a 1.5- to 1.6-kb transcript in all tissues examined and in 8 brain regions.GENE STRUCTURE
Winfield et al. (1997) determined that the SCAMP3 gene, which they called PROPIN1, contains 9 exons and spans 6.4 kb of genomic DNA.MAPPING
Winfield et al. (1997) mapped the SCAMP3 gene to chromosome 1q21 by sequence analysis of a YAC clone mapped to 1q21. ... More on the omim web site
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 606913 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).