C-X-C motif chemokine 5 (CXCL5)
The protein contains 114 amino acids for an estimated molecular weight of 11972 Da.
Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. (updated: Sept. 12, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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No binding partner found
Biological Process
Antimicrobial humoral immune response mediated by antimicrobial peptide
Cell-cell signaling
Cellular response to lipopolysaccharide
Chemokine-mediated signaling pathway
Chemotaxis
G protein-coupled receptor signaling pathway
Immune response
Inflammatory response
Leukocyte chemotaxis
Neutrophil chemotaxis
Positive regulation of cell population proliferation
Signal transduction
The reference OMIM entry for this protein is 600324
Chemokine, cxc motif, ligand 5; cxcl5
Small inducible cytokine subfamily b, member 5; scyb5
Neutrophil-activating peptide ena-78; ena78
Lipopolysaccharide-induced cxc chemokine; lix
CLONING
Epithelial cell-derived neutrophil-activating peptide ENA78 is an inflammatory chemokine that is produced concomitantly with interleukin-8 (IL8; 146930) in response to stimulation with either interleukin-1 (IL1B; 147720) or tumor necrosis factor-alpha (TNFA; 191160). Chang et al. (1994) identified a full-length ENA78 cDNA and isolated its genomic clone. Rovai et al. (1997) cloned the human ENA78 gene (which is also symbolized SCYB5) as well as GCP2 (SCYB6; 138965). Both coding and noncoding portions of the GCP2 gene share very high nucleotide similarity to ENA78, except for the occurrence of a long interspersed sequence 5-prime of the GCP2 gene. Despite 85% identity of the first 270 nucleotides 5-prime of the transcription start sites, GCP2 and the other CXC chemokine gene ENA78 showed cell-specific differences in regulation.GENE STRUCTURE
Chang et al. (1994) determined that the ENA78 gene consists of 4 exons and 3 introns, with a structure resembling that of the IL8 gene. The transcriptional initiation site was mapped to a position 96 bp upstream from the translation initiation site.GENE FAMILY
Chemokines are a group of small (approximately 8-14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC, based on the arrangement of the first 2 of the 4 conserved cysteine residues; the 2 cysteines are separated by a single amino acid in CXC chemokines and are adjacent in CC chemokines. CXC chemokines are further subdivided into ELR and non-ELR types based on the presence or absence of a glu-leu-arg sequence adjacent and N terminal to the CXC motif (summary by Strieter et al., 1995; Zlotnik and Yoshie, 2000).MAPPING
By PCR analysis and mapping of YAC clones, O'Donovan et al. (1999) localized a number of CXC chemokine genes to chromosome 4q12-q21. They proposed that the order in this region is centromere--IL8--GRO1 (155730)/PPBP (121010)/PF4 (173460)--SCYB5/SCYB6--GRO2 (139110)/GRO3 (139111)--SCYB11 (604852)--SCYB10 (147310)--MIG (601704)--telomere. The SCYB5 gene was localized to 4q12-q13. By fluorescence in situ hybridization, Chang et al. (1994) mapped the ENA78 gene to 4q13-q21, the same region to which several other inflammatory cytokine genes have been mapped. Chang et al. (1994) found that even though the ENA78 and IL8 genes share great similarity in genomic structure and chromosome location, they appear to be regulated by different mechanisms. ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 600324 was added.
Feb. 22, 2019: Protein entry updated
Automatic update: comparative model was added.
Feb. 22, 2019: Protein entry updated
Automatic update: model status changed
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).