Nucleolar protein 6 (NOL6)
The protein contains 1146 amino acids for an estimated molecular weight of 127593 Da.
No function (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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| Variant | Description |
|---|---|
| dbSNP:rs10971523 | |
| dbSNP:rs35135082 |
The reference OMIM entry for this protein is 611532
Nucleolar protein 6; nol6
Nucleolar rna-associated protein; nrap
Utp22, s. cerevisiae, homolog of; utp22
CLONING
By screening a mouse embryonic stem cell cDNA library with a partial Nol6 clone, followed by 5-prime RACE, database analysis, and RT-PCR of human hepatic cell line RNA, Utama et al. (2002) cloned full-length human and mouse NOL6, which they called NRAP. They identified NOL6-alpha, -beta, and -gamma isoforms that contain 1,146, 1,007, and 699 amino acids, respectively. NOL6-alpha shares 88% amino acid identity with mouse Nol6-alpha. Both human and mouse coding regions are GC-rich and share weak homology to a PAP/25A core domain, which is found in poly(A) polymerases (605553). NOL6 contains potential nuclear localization signals and has several potential phosphorylation sites. Immunohistochemical and immunofluorescence studies localized Nol6 expression within the nucleolus in a variety of rodent cell lines, although expression was not specifically localized to rRNA transcription sites. Nol6 localized to the condensed chromosomes during mitosis in a pattern similar to that of B23/nucleophosmin (NPM1; 164040). In situ hybridization studies in mouse embryos showed ubiquitous Nol6 expression during development. Immunoblot studies of mouse tissues detected strong expression in spleen, testis, colon, kidney, stomach, and brain, with moderate expression in lung, liver, and small intestine, and low expression in heart and skeletal muscle. By database analysis, Bernstein et al. (2004) identified NOL6 as the human ortholog of yeast Utp22. Utp22 is a component of a yeast ribonucleoprotein required for biogenesis of the 18S rRNA.GENE FUNCTION
Utama et al. (2002) showed that both actinomycin D and RNase treatment disrupted Nol6 nucleolar localization while cyclohexamide had no effect. They suggested that Nol6 interacts either directly or indirectly with the pre-rRNA transcript.GENE STRUCTURE
Utama et al. (2002) determined that the NOL6 gene contains 26 exons spanning 11.4 kb. Alternative splicing excludes exons 24 and 25 in NOL6-beta and skips exons 16 to 25 in the NOL6-gamma isoform.MAPPING
By genomic sequence analysis, Utama et al. (2002) mapped the NOL6 gene to chromosome 9p13. ... More on the omim web site
Oct. 20, 2018: Protein entry updated
Automatic update: OMIM entry 611532 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).