Kinesin-like protein KIF13B (KIF13B)
The protein contains 1826 amino acids for an estimated molecular weight of 202789 Da.
Involved in reorganization of the cortical cytoskeleton. Regulates axon formation by promoting the formation of extra axons. May be functionally important for the intracellular trafficking of MAGUKs and associated protein complexes. (updated: Oct. 10, 2018)
Protein identification was indicated in the following studies:
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
(right-click above to access to more options from the contextual menu)
| Variant | Description |
|---|---|
| dbSNP:rs17526980 |
Biological Process
Microtubule-based movement
Protein targeting
Regulation of axonogenesis
Signal transduction
T cell activation
Cellular Component
Axon
Cytoplasm
Cytosol
Kinesin complex
Microtubule
Microvillus
Paranode region of axon
The reference OMIM entry for this protein is 607350
Kinesin family member 13b; kif13b
Guanylate kinase-associated kinesin; gakin
Kiaa0639
CLONING
By randomly sequencing clones obtained from a size-fractionated brain cDNA library, Ishikawa et al. (1998) cloned a partial cDNA encoding KIF13B, which they called KIAA0639. RT-PCR detected high levels of KIF13B expression in all tissues examined. Hanada et al. (2000) identified KIF13B, which they called GAKIN, as a 250-kD phosphoprotein that interacted with DLG1 (601014) in Jurkat T cells. By peptide sequencing, EST database searching, amplification, and conventional cloning, they obtained a full-length KIF13B cDNA from a fetal brain cDNA library. The deduced 1,826-amino acid protein has a calculated molecular mass of 203 kD. KIF13B contains a consensus ATP/GTP-binding motif within its N-terminal motor domain; a stalk domain containing several short alpha-helical segments; and a CAP-gly motif in its C terminus characteristic of microtubule-based cytoskeleton components. KIF13B has several putative serine/threonine phosphorylation sites and a putative tyrosine phosphorylation site. The N-terminal motor domain of KIF13B shares 98% identity with the motor domain of mouse Kif13b. Northern blot analysis revealed a single 8.5-kb transcript in all tissues tested, with highest abundance in kidney, pancreas, brain, and testis. Western blot analysis of Jurkat T cells revealed an apparent molecular mass of 250 kD, and immunolocalization revealed a punctate, cytoplasmic distribution in resting T cells.NOMENCLATURE
Lawrence et al. (2004) presented a standardized kinesin nomenclature based on 14 family designations. Under this system, KIF13B belongs to the kinesin-3 family.GENE FUNCTION
Using in vitro pull-down assays and coimmunoprecipitation experiments, Hanada et al. (2000) confirmed the interaction between KIF13B and DLG1. Mutation analysis indicated that a segment of the KIF13B stalk domain in close proximity to the motor domain is required for binding with DLG1. KIF13B also bound the guanylate kinase-like domain of PSD95 (DLG4; 602887), but not that of p55 (MPP1; 305360).MAPPING
By radiation hybrid analysis, Ishikawa et al. (1998) mapped the KIF13B gene to chromosome 8. Gross (2012) mapped the KIF13B gene to chromosome 8p12 based on an alignment of the KIF13B sequence (GenBank GENBANK AF279865) with the genomic sequence (GRCh37). ... More on the omim web site
June 30, 2020: Protein entry updated
Automatic update: OMIM entry 607350 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).