60S acidic ribosomal protein P0 (RPLP0)

The protein contains 317 amino acids for an estimated molecular weight of 34274 Da.

 

Ribosomal protein P0 is the functional equivalent of E.coli protein L10. (updated: April 1, 2015)

Protein identification was indicated in the following studies:

  1. Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
  2. Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
  3. Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
  4. Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
  5. D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
  6. Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.

Methods

The following articles were analysed to gather the proteome content of erythrocytes.

The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.

PublicationIdentification 1Uniprot mapping 2Not mapped /
Obsolete		TrEMBLSwiss-Prot
Goodman (2013)2289 (gene list)227853205992269
Lange (2014)123412347281224
Hegedus (2015)2638262202352387
Wilson (2016)165815281702911068
d'Alessandro (2017)18261817201815
Bryk (2017)20902060101081942
Chu (2018)18531804553621387

1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry

The compilation of older studies can be retrieved from the Red Blood Cell Collection database.

The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.

No sequence conservation computed yet.
Protein sequence (FASTA)
Protein coevolution profile (FreeContact)
Multiple Sequence Alignment (Muscle)

Interpro domains
Total structural coverage: 61%
Model score: 100
MODL_I-TASSER-3.0

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The reference OMIM entry for this protein is 180510

Ribosomal phosphoprotein, large, p0; rplp0
Ribosomal phosphoprotein, acidic, p0

DESCRIPTION

Acidic ribosomal proteins, called A-proteins or P-proteins, are generally present in multiple copies on the ribosome and have isoelectric points in the range of pH 3 to 5, in contrast to most ribosomal proteins, which are single copy and basic. A-proteins have hydrophobic amino acid compositions, notably about 20% alanine.

CLONING

Rich and Steitz (1987) described the isolation and analysis of 3 cDNA molecules that encode the human P-proteins, P2 (180530), P1 (180520), and P0. The human P1 and P2 cDNA nucleotide sequences and deduced amino acid sequences of the proteins are very similar to the sequences that have been determined for the corresponding rat, shrimp, and yeast proteins and cDNAs. P0 sequences had not previously been described. To demonstrate that the coding sequences are full length, Rich and Steitz (1987) transcribed the P0, P1, and P2 cDNAs in vitro. The P0, P1, and P2 proteins produced were serologically and electrophoretically identical to P-proteins extracted from HeLa cells. The deduced P0 protein contains 317 amino acids and is the largest of the 3 proteins. Northern blot analysis revealed expression of a 1.2-kb transcript in HeLa cells.

MAPPING

The International Radiation Hybrid Mapping Consortium mapped the RPLP0 gene to chromosome 12 (TMAP D12S1979). ... More on the omim web site

Subscribe to this protein entry history

May 12, 2019: Protein entry updated
Automatic update: model status changed

Nov. 16, 2018: Protein entry updated
Automatic update: model status changed

Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated

Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated

Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated

Oct. 26, 2017: Protein entry updated
Automatic update: model status changed

March 25, 2017: Additional information
No protein expression data in P. Mayeux work for RPLP0

March 15, 2016: Protein entry updated
Automatic update: OMIM entry 180510 was added.

Jan. 24, 2016: Protein entry updated
Automatic update: model status changed