Protein SLFN14 (SLFN14)
The protein contains 912 amino acids for an estimated molecular weight of 103907 Da.
Shows no ribosome-associated and endoribonuclease activities.', 'Displays polysome-associated endoribonuclease activity towards mRNAs and rRNAs (PubMed:25996083). May play a role in RNA surveillance pathways by recognizing stalled ribosomes and triggering endonucleolytic cleavage of aberrant mRNAs (Probable). Cleaves different types of rRNAs and mRNAs in a magnesium- and manganese-dependent and ATP-independent manner (By similarity). Involved in correct maturation of megakaryocytes and especially important for proplatelet extension. (updated: Dec. 11, 2019)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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No binding partner found
The reference OMIM entry for this protein is 614958
Schlafen family, member 14; slfn14
DESCRIPTION
The Schlafen (SFLN) family contains 9 mouse and 6 human genes, including SLFN14, that encode proteins with a conserved N terminus containing a putative AAA ATPase domain. Longer SLFN proteins have C-terminal motifs resembling DNA/RNA helicase domains. Studies in mouse have implicated SLFN proteins in regulation of cell growth and T-cell development (summary by Geserick et al. (2004) and Li et al. (2012)).MAPPING
Gross (2012) mapped the SLFN14 gene to chromosome 17q12 based on an alignment of the SLFN14 sequence (GenBank GENBANK BC140847) with the genomic sequence (GRCh37). Bustos et al. (2009) found that the SLFN genes are clustered within the same orthologous region flanked by the UNC45 (UNC45B; 611220) and PEX12 (601758) genes in all mammalian genomes examined. The human and mouse SLFN clusters are located on chromosomes 17 and 11, respectively.EVOLUTION
Bustos et al. (2009) used genomic and phylogenetic studies to investigate the evolution and role of the SLFN gene family. They found that the SLFN family is widely distributed in mammals, with 4 major clades that underwent lineage-specific expansions or contractions in various orders, including primates and rodents. Bustos et al. (2009) also identified SLFN family members in Chondrichthyes and Amphibia. They found evidence that positive selection has acted on many SLFN genes. A member of the SLFN family was horizontally transferred from mouse to orthopoxviruses, where the authors hypothesized it plays a role in allowing the virus to survive host defense mechanisms. The viral SLFN sequences are evolving under purifying selection, indicating their functional relevance. Van Zuylen et al. (2011) noted that only 2 SLFN family members, SLFN5 (614952) and SLFN14, have direct orthologs in mouse and human, consistent with rapid evolution of the SLFN family across mammalian species. Human SLFN12 (614956) and SLFN12L (614957) have 4 orthologs in mice (Slfn1, Slfn2, Slfn3, and Slfn4), and human SLFN11 (614953) and SLFN13 (614957) have 3 orthologs in mice (Slfn8, Slfn9, and Slfn10). ... More on the omim web site
Jan. 22, 2020: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 10, 2018: Protein entry updated
Automatic update: Entry updated from uniprot information.
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 614958 was added.