40S ribosomal protein S28 (RPS28)
The protein contains 69 amino acids for an estimated molecular weight of 7841 Da.
No function (updated: Aug. 16, 2004)
Protein identification was indicated in the following studies:
- Wilson and co-workers. (2016) Comparison of the Proteome of Adult and Cord Erythroid Cells, and Changes in the Proteome Following Reticulocyte Maturation. Mol Cell Proteomics. 15(6), 1938-1946.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- Chu and co-workers. (2018) Quantitative mass spectrometry of human reticulocytes reveal proteome-wide modifications during maturation. Br J Haematol. 180(1), 118-133.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Cytoplasmic translation
Maturation of SSU-rRNA
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
Ribosomal small subunit assembly
Ribosomal small subunit biogenesis
Ribosome biogenesis
RRNA processing
SRP-dependent cotranslational protein targeting to membrane
Translation
Translational initiation
Viral transcription
The reference OMIM entry for this protein is 603685
Ribosomal protein s28; rps28
DESCRIPTION
The RPS28 gene encodes a ribosomal protein likely involved in ribosome biogenesis (summary by Gripp et al., 2014). The mammalian ribosome is composed of 4 RNA species (see 180450) and approximately 80 different proteins.CLONING
Chan et al. (1991) cloned and characterized a rat ribosomal protein S28 (Rps28) cDNA. They deposited the sequence of a human RPS28 cDNA in GenBank (GENBANK L05091).MAPPING
By somatic cell hybrid and radiation hybrid mapping analyses, Kenmochi et al. (1998) mapped the human RPS28 gene to 19p13.2 (GenBank GENBANK AB007164).MOLECULAR GENETICS
In 2 unrelated girls with Diamond-Blackfan anemia-15 and mandibulofacial dysostosis (DBA15; 606164), Gripp et al. (2014) identified the same de novo heterozygous mutation affecting the translation initiation codon of the RPS28 gene (c.1A-G; 603685.0001), predicted to result in haploinsufficiency. Functional studies of the variant were not performed, but Gripp et al. (2014) noted that RPS28 interacts with other RPS proteins to generate ribosomes, and that the mutation may be comparable to the loss of RPS19 (603474), which is mutated in DBA1 (105650). ... More on the omim web site
June 29, 2020: Protein entry updated
Automatic update: OMIM entry 603685 was added.
Oct. 19, 2018: Additional information
Initial protein addition to the database. This entry was referenced in Bryk and co-workers. (2017).