Heterogeneous nuclear ribonucleoprotein H (HNRNPH1)
The protein contains 449 amino acids for an estimated molecular weight of 49229 Da.
This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG). (updated: March 4, 2015)
Protein identification was indicated in the following studies:
- Goodman and co-workers. (2013) The proteomics and interactomics of human erythrocytes. Exp Biol Med (Maywood) 238(5), 509-518.
- Lange and co-workers. (2014) Annotating N termini for the human proteome project: N termini and Nα-acetylation status differentiate stable cleaved protein species from degradation remnants in the human erythrocyte proteome. J Proteome Res. 13(4), 2028-2044.
- Hegedűs and co-workers. (2015) Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications. Database (Oxford) 1-8.
- Bryk and co-workers. (2017) Quantitative Analysis of Human Red Blood Cell Proteome. J Proteome Res. 16(8), 2752-2761.
- D'Alessandro and co-workers. (2017) Red blood cell proteomics update: is there more to discover? Blood Transfus. 15(2), 182-187.
Methods
The following articles were analysed to gather the proteome content of erythrocytes.
The gene or protein list provided in the studies were processed using the ID mapping API of Uniprot in September 2018. The number of proteins identified and mapped without ambiguity in these studies is indicated below.
Only Swiss-Prot entries (reviewed) were considered for protein evidence assignation.
| Publication | Identification 1 | Uniprot mapping 2 | Not mapped / Obsolete | TrEMBL | Swiss-Prot |
|---|---|---|---|---|---|
| Goodman (2013) | 2289 (gene list) | 2278 | 53 | 20599 | 2269 |
| Lange (2014) | 1234 | 1234 | 7 | 28 | 1224 |
| Hegedus (2015) | 2638 | 2622 | 0 | 235 | 2387 |
| Wilson (2016) | 1658 | 1528 | 170 | 291 | 1068 |
| d'Alessandro (2017) | 1826 | 1817 | 2 | 0 | 1815 |
| Bryk (2017) | 2090 | 2060 | 10 | 108 | 1942 |
| Chu (2018) | 1853 | 1804 | 55 | 362 | 1387 |
1 as available in the article and/or in supplementary material
2 uniprot mapping returns all protein isoforms as one entry
The compilation of older studies can be retrieved from the Red Blood Cell Collection database.
The data and differentiation stages presented below come from the proteomic study and analysis performed by our partners of the GReX consortium, more details are available in their published work.
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Biological Process
Fibroblast growth factor receptor signaling pathway
Gene expression
MRNA splicing, via spliceosome
Regulation of RNA splicing
RNA metabolic process
RNA processing
RNA splicing
Cellular Component
Catalytic step 2 spliceosome
Cytosol
Membrane
Nucleoplasm
Nucleus
Ribonucleoprotein complex
Molecular Function
Identical protein binding
Nucleotide binding
Poly(A) RNA binding
Poly(U) RNA binding
RNA binding
The reference OMIM entry for this protein is 601035
Heterogeneous nuclear ribonucleoprotein h1; hnrnph1
Hnrph1
Heterogeneous nuclear ribonucleoprotein h
CLONING
Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that bind heterogeneous nuclear RNA (hnRNA), the transcripts produced by RNA polymerase II (see 180660). More than 20 such proteins have been described and designated with letters from A to U. By molecular cDNA cloning, 2-dimensional gel immunoblotting, and amino acid microsequencing, Honore et al. (1995) identified 3 sequence-unique and distinct proteins that constitute a subfamily of ubiquitously expressed hnRNPs. The identity between hnRNPs H and H-prime (300610) was 96%, between H and F (601037), 78%, and between H-prime and F, 75%.MAPPING
By fluorescence in situ hybridization, Honore et al. (1995) mapped the HNRNPH1 gene to chromosome 5q35.3.GENE FUNCTION
Masuda et al. (2008) identified a key nucleotide in an intron of the CHRNA1 gene (100690) that is part of an intronic splicing silencer (ISS) sequence targeted by hnRNP H and suppresses inclusion of an alternative exon in the CHRNA1 gene. Analysis of the human genome suggested that the hnRNP H-binding motif 'UGGG' is overrepresented close to the 3-prime ends of introns. Masuda et al. (2008) found that alternative exons of several other genes, including GRIP1 (604597), FAS (TNFRSF6; 134637), VPS13C (608879), and NRCAM (601581) are downregulated by hnRNP H. These findings suggest that presence of the hnRNP H-binding motif close to the 3-prime end of an intron is an essential, and perhaps underestimated, splicing regulator of the downstream exon. ... More on the omim web site
May 12, 2019: Protein entry updated
Automatic update: model status changed
Nov. 17, 2018: Protein entry updated
Automatic update: model status changed
Feb. 2, 2018: Protein entry updated
Automatic update: Uniprot description updated
Dec. 19, 2017: Protein entry updated
Automatic update: Uniprot description updated
Nov. 23, 2017: Protein entry updated
Automatic update: Uniprot description updated
Oct. 27, 2017: Protein entry updated
Automatic update: model status changed
March 16, 2016: Protein entry updated
Automatic update: OMIM entry 601035 was added.
Jan. 24, 2016: Protein entry updated
Automatic update: model status changed